中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (8): 837-844.doi: 10.4103/1673-5374.131600

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

脂肪源性基质细胞体外诱导分化为星形胶质细胞障碍

  

  • 收稿日期:2014-03-28 出版日期:2014-04-25 发布日期:2014-04-25

Apoptosis is an obstacle to the differentiation of adipose-derived stromal cells into astrocytes

Xiaodong Yuan 1, Qiaoyu Sun 1, Ya Ou 1, Shujuan Wang 1, Wenli Zhang 2, Hongliang Deng 1, Xiaoying Wu 1, Lili Zhang 1   

  1. 1 Department of Neurology, Kailuan General Hospital, Hebei United University, Tangshan, Hebei Province, China
    2 Department of Electron Microscopy, Hebei United University, Tangshan, Hebei Province, China
  • Received:2014-03-28 Online:2014-04-25 Published:2014-04-25
  • Contact: Xiaodong Yuan, Department of Neurology, Kailuan General Hospital, Hebei United University, Tangshan 063000, Hebei Province, China, yxd68@sohu.com.

摘要:

既往研究发现脂肪源性基质细胞经化学诱导后,分化的神经细胞存活时间较短,原因有待证实。实验应用化学诱导剂诱导成人脂肪基质细胞分化为星形胶质细胞,经MTT和流式细胞仪检测,随诱导时间的延长,分化细胞中细胞凋亡率逐渐增加,存活细胞数量逐渐减少。免疫组化染色显示,随着诱导时间的延长,细胞中GFAP,caspase-3和caspase-9阳性细胞数量逐渐增加。透射电镜观察到分化后的细胞部分出现典型的细胞凋亡特征。表明caspase依赖性细胞凋亡是脂肪源性基质细胞分化为星形胶质细胞的障碍,提示抑制这种细胞凋亡可能是改善诱导星形胶质细胞效果的重要措施。

关键词: 神经再生, 成人脂肪源性基质细胞, 细胞凋亡, caspase依赖性细胞凋亡, 细胞分化, 星形胶质细胞, caspase-9, caspase-3

Abstract:

Previous studies have demonstrated that nerve cells differentiated from adipose-derived stromal cells after chemical induction have reduced viability; however, the underlying mechanisms remained unclear. In this study, we induced the differentiation of adult adipose-derived stromal cells into astrocytes using chemical induction. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry showed that, with increasing induction time, the apoptotic rate gradually increased, and the number of living cells gradually decreased. Immunohistochemical staining demonstrated that the number of glial fibrillary acidic protein-, caspase-3- and caspase-9-positive cells gradually increased with increasing induction time. Transmission electron microscopy revealed typical signs of apoptosis after differentiation. Taken together, our results indicate that caspase-dependent apoptosis is an obstacle to the differentiation of adipose-derived stromal cells into astrocytes. Inhibiting apoptosis may be an important strategy for increasing the efficiency of induction.

Key words: nerve regeneration, adult adipose-derived stromal cells, cell apoptosis, caspase-dependent apoptosis, cell differentiation, astrocytes, caspase-9, caspase-3, neural regeneration