中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (10): 1063-1067.doi: 10.4103/1673-5374.133172

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

趋化因子受体4基因沉默可抑制神经母细胞瘤的体外侵袭

  

  • 收稿日期:2014-04-05 出版日期:2014-05-26 发布日期:2014-05-26
  • 基金资助:

    国家自然科学基金(30872702)资助

Chemokine receptor 4 gene silencing blocks neuroblastoma metastasis in vitro

Xin Chen  1, Yongjie Zhu  1, Lulu Han  2, Hongting Lu  1, Xiwei Hao 1, Qian Dong 1   

  1. 1 Department of Pediatric Surgery, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China
    2 Operating Room, the Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China
  • Received:2014-04-05 Online:2014-05-26 Published:2014-05-26
  • Contact: Qian Dong, Department of Pediatric Surgery, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China, dong.qian@sohu.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81272986 and the Natural Science Foundation of Shandong Province, No. ZR2011HZ002.

摘要:

实验构建了趋化因子受体4小分子干扰RNA表达载体,观察其对体外神经母细胞瘤体外侵袭能力的影响。选择可表达趋化因子受体4的神经母细胞瘤SH-SY5Y细胞系,设计合成人趋化因子受体4基因不同靶点的能编码siRNA的3条双链DNA序列,克隆到真核表达载体pSilencerTM neo中构建趋化因子受体4-小分子干扰RNA表达载体,体外脂质体介导转染至SH-SY5Y细胞,沉默趋化因子受体4基因的表达。发现与正常培养的神经母细胞瘤细胞相比,转染构建趋化因子受体4-小分子干扰RNA表达载体后的神经母细胞瘤细胞趋化因子受体4 mRNA和蛋白表达均降低,体外细胞侵袭能力降低。说明下调趋化因子受体4的表达,可抑制神经母细胞瘤的体外侵袭。

关键词: 神经再生, 趋化因子受体4, 小干扰RNA, 神经母细胞瘤, 侵袭, Transwell小室, 脂质体, 国家自然科学基金

Abstract:

This study investigated the effects of small interfering RNA (siRNA)-mediated silencing of chemokine receptor 4 (CXCR4) on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was significantly suppressed in transfected cells by all three sequence-specific siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was significantly decreased following transfection with CXCR4-specific siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.

Key words: nerve regeneration, chemokine receptor 4, small interfering RNA, neuroblastoma, invasion, Transwell chamber, liposome, NSFC grant, neural regeneration