中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (12): 1217-1221.doi: 10.4103/1673-5374.135327

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

长时间阳性阻滞电刺激兔骶神经根不会造成其损伤

  

  • 收稿日期:2014-05-12 出版日期:2014-06-24 发布日期:2014-06-24
  • 基金资助:

    吉林省科委国际合作项目资助(20100735)

Prolonged electrical stimulation causes no damage to sacral nerve roots in rabbits

Peng Yan 1, 2, Xiaohong Yang 1, Xiaoyu Yang 1, Weidong Zheng 2, Yunbing Tan 2   

  1. 1 Jilin University, Changchun, Jilin Province, China
    2 People’s Hospital of Jilin Province, Changchun, Jilin Province, China
  • Received:2014-05-12 Online:2014-06-24 Published:2014-06-24
  • Contact: Xiaohong Yang, M.D., Jilin University, Changchun 130021, Jilin Province, China, 13596197541@139.com. Xiaoyu Yang, M.D., Jilin University, Changchun 130021, Jilin Province, China, yangxiaoyu88@sina.com.
  • Supported by:

    This study was supported by the International Cooperation Projects of Jilin Province  Science and Technology Commission, No. 20100735.

摘要:

我们前期研究发现应用阳极阻滞技术电刺激截瘫兔骶神经根可使其产生生理性排尿,重建膀胱排尿功能,但长时间的阳极阻滞电刺激是否会对骶神经根产生损伤,还需进行验证。实验将新西兰兔脊髓T9-10横建立完全脊髓损伤模型后,以短时间连续电刺激的方式模拟临床中长时间慢性电刺激。结果发现与正常新西兰兔相比,脊髓损伤模型兔经电刺激后骶神经前根中神经细胞结构无明显改变,且其中细胞凋亡相关蛋白Bax,Bcl-2和Caspase-3的表达也未发生明显改变。提示兔骶神经根神经元细胞能很好耐受这一刺激量而不会出现损伤,即使是长时间的阳极阻滞电刺激对骶神经根也不会造成损伤现象。

关键词: 神经再生, 脊髓神经损伤, 骶神经根, 电刺激, 阳极阻滞, 脊髓重建, 膀胱功能, 神经假体

Abstract:

Previous studies have shown that, anode block electrical stimulation of the sacral nerve root can produce physiological urination and reconstruct urinary bladder function in rabbits. However, whether long-term anode block electrical stimulation causes damage to the sacral nerve root remains unclear, and needs further investigation. In this study, a complete spinal cord injury model was established in New Zealand white rabbits through T9–10 segment transection. Rabbits were given continuous electrical stimulation for a short period and then chronic stimulation for a longer period. Results showed that compared with normal rabbits, the structure of nerve cells in the anterior sacral nerve roots was unchanged in spinal cord injury rabbits after electrical stimulation. There was no significant difference in the expression of apoptosis-related proteins such as Bax, Caspase-3, and Bcl-2. Experimental findings indicate that neurons in the rabbit sacral nerve roots tolerate electrical stimulation, even after long-term anode block electrical stimulation.

Key words: nerve regeneration, spinal cord injury, sacral nerve root, electrical stimulation, anode  , block, spinal cord reconstruction, bladder function, nerve prosthesis, neural regeneration

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