中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (13): 1267-1274.doi: 10.4103/1673-5374.137573

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

小动物PET成像研究传统复方中药通心络的神经保护作用

  

  • 收稿日期:2014-06-18 出版日期:2014-07-11 发布日期:2014-07-11

Neuroprotective effect of the traditional Chinese herbal formula Tongxinluo: a PET imaging study in rats

Xiao Cheng 1, Haoxuan Luo 2, Lihua Zhou 2, Lixin Wang 1, Jingbo Sun 1, Yan Huang 1, Enli Luo 1, Yefeng Cai 1   

  1. 1 Encephalopathy Center, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, Guangdong Province, China
    2 Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong Province, China
  • Received:2014-06-18 Online:2014-07-11 Published:2014-07-11
  • Contact: Yefeng Cai, M.D., Ph.D., Encephalopathy Center, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120,Guangdong Province, China, cheng888@qq.com.
  • Supported by:

    This study was financially supported by the National Natural Science Foundation of China, No. 81173435, 81303115, the Natural Science Foundation of Guangdong Province, No. S2013040016915, and the Postdoctoral Foundation of China, No. BBK42913K09, 201003345.

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摘要：

通心络在中国已广泛用于急性脑卒中的治疗及神经保护。但通心络对脑缺血再灌注是否有神经保护作用的小动物正电子发射断层扫描研究尚缺乏。由此，实验在建立大脑中动脉阻塞大鼠模型前给予连续5d的通心络悬液灌胃干预，对脑缺血再灌注后1和2周的小动物18F-氟脱氧葡萄糖正电子发射断层扫描检测，见通心络预处理可提高脑缺血区葡萄糖代谢水平；尼氏染色结果显示，通心络预处理可使脑缺血再灌注后2周大脑前额叶皮质神经丢失减少。通心络预处理还可使脑缺血再灌注大鼠神经功能缺损减轻，脑梗死体积减小。实验结果证实，通心络预处理对脑缺血再灌注损伤有神经保护作用；小动物18F-氟脱氧葡萄糖正电子发射断层扫描是研究中药分子药理学的理想检测工具。

关键词: 神经再生, 脑缺血再灌注, 通心络, 大脑中动脉阻塞, 正电子发射计算机断层扫描, 神经保护

Abstract:

oprotection. However, there are few positron emission tomography (PET) studies on the neuroprotective effect of Tongxinluo on cerebral ischemia/reperfusion in small animals. In the present study, Tongxinluo superfine powder suspension or its vehicle was administered intragastrically to rats for 5 successive days before middle cerebral artery occlusion. 18F-fluorodeoxyglucose (FDG) small animal PET imaging showed that at 1 and 2 weeks after cerebral ischemia/reperfusion, glucose metabolism in the ischemic area was greater in rats that had received Tongxinluo than in those that had received the vehicle. Nissl staining showed that 2 weeks after cerebral ischemia/reperfusion, there was less neuronal loss in the prefrontal cortex in Tongxinluo-treated rats than in controls. In addition, Tongxinluo-treated animals showed better neurologic function and lower cerebral infarct volume than rats that received the vehicle. These findings suggest that Tongxinluo exhibits neuroprotective effects in cerebral ischemia/reperfusion injury and demonstrates that 18F-FDG small animal PET imaging is a useful tool with which to study the molecular pharmacology of traditional Chinese medicine.

Key words:  nerve regeneration, cerebral ischemia/reperfusion, Tongxinluo capsule, middle cerebral artery occlusion, positron emission tomography, neuroprotection, NSFC grant, neural regeneration