中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (17): 1592-1598.doi: 10.4103/1673-5374.141784

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

黄芩茎叶黄酮为何能降低缺氧性大脑皮质神经元的凋亡?

  

  • 收稿日期:2014-07-05 出版日期:2014-09-16 发布日期:2014-09-16

Mechanisms underlying attenuation of apoptosis of cortical neurons in the hypoxic brain by flavonoids from the stems and leaves of Scutellaria baicalensis Georgi

Guangxin Miao 1, Hongxiang Zhao 1, Ke Guo 1, Jianjun Cheng 1, Shufeng Zhang 1, Xiaofeng Zhang 1, Zhenling Cai 2, Hong Miao 1, Yazhen Shang 1   

  1. 1 Institute of Traditional Chinese Medicine, Chengde Medical College / Key Subject Construction Project of Hebei Provincial College / Hebei
    Province Key Laboratory of Traditional Chinese Medicine Research and Development, Chengde, Hebei Province, China
    2 Department of Anesthesiology, Affiliated Hospital of Chengde Medical College, Chengde, Hebei Province, China
  • Received:2014-07-05 Online:2014-09-16 Published:2014-09-16
  • Contact: Yazhen Shang, M.D., Institute of Traditional Chinese Medicine, Chengde Medical College / Key Subject Construction Project of Hebei Provincial College / Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development, Chengde 067000, Hebei Province, China, shangyz1018@sina.com.

摘要:

来自植物黄芩茎叶的提取的黄酮是一种抗氧化剂,对记忆障碍和神经损伤有显著的改善作用。实验利用黄酮的这一作用,建立缺氧剂氰化钾损伤大鼠原代大脑皮质细胞建立神经元凋亡体外模型,旨在揭示18.98, 37.36和75.92 μg/mL黄芩茎叶黄酮对神经元凋亡的抑制作用。结果发现,黄芩茎叶黄酮可显著增加暴露于氰化钾大鼠大脑皮质神经元的存活率,抑制细胞凋亡和丙二醛的过度生成、增加超氧化物歧化酶、谷胱甘肽过氧化物酶和Na+-K+-ATP酶的活性。说明黄芩茎叶黄酮可能通过调节氰化钾导致的脑内氧化应激和Na+-K+-ATP酶异常的途径对神经元起保护作用。

关键词: 脑损伤, 黄芩, 黄酮, 氰化钾, 细胞凋亡, 乳酸脱氢酶, 氧化应激, 丙二醛, 超氧化物歧化酶, 谷胱甘肽过氧化物酶, Na+-K+- ATP酶

Abstract:

Flavonoids from the stems and leaves of Scutellaria baicalensis Georgi, an antioxidant, markedly improve memory impairments and neuronal injuries. In the present study, primary cortical neurons of rats were exposed to potassium cyanide to establish a model of in vitro neural cell apoptosis. Inhibition of apoptosis by flavonoids from the stems and leaves of Scutellaria baicalensis Georgi at concentrations of 18.98, 37.36, and 75.92 μg/mL was detected using this model. These flavonoids dramatically increased cell survival, inhibited cell apoptosis and excessive production of malondialdehyde, and increased the activities of superoxide dismutase, glutathione peroxidase, and Na+-K+-ATPase in primary cortical neurons exposed to potassium cyanide. The flavonoids from the stems and leaves of Scutellaria baicalensis Georgi were originally found to have a polyhydric structure and to protect against cerebral hypoxia in in vitro and in vivo models, including hypoxia induced by potassium cyanide or cerebral ischemia. The present study suggests that flavonoids from the stems and leaves of Scutellaria baicalensis Georgi exert neuroprotective effects via modulation of oxidative stress, such as malondialdehyde, superoxide dismutase, glutathione peroxidase and Na+-K+-ATPase disorders induced by potassium cyanide.

Key words: nerve regeneration, brain injury, Scutellaria baicalensis Georgi, flavonoids, potassium cyanide, apoptosis, oxidative stress, Na+-K+-ATPase, neural regeneration