中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (22): 2002-2007.doi: 10.4103/1673-5374.145384

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

中高浓度异丙酚和瑞芬太尼可影响神经干细胞/祖细胞的增殖分化

  

  • 收稿日期:2014-09-11 出版日期:2014-11-25 发布日期:2014-11-25

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells

Qing Li 1, 2, Jiang Lu 1, 2, Xianyu Wang 1, 2   

  1. 1 Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China
    2 Anesthesiology Research Institude, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China
  • Received:2014-09-11 Online:2014-11-25 Published:2014-11-25
  • Supported by:

    This study was supported by the Natural Science Foundation of Hubei Province of China, No. 2012FFC060; the Natural Science Foundation of Hubei University of Medicine in China, No. 2011QDZR-2; the Provincial Key Disciplines Foundation of Hubei Province of China, No. 2014XKJSSJ04.

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摘要：

有研究提示，麻醉药物异丙酚和瑞芬太尼可通过激活γ-氨基丁酸A型受体或通过减少睾酮水平从而影响神经干细胞/祖细胞内钙离子浓度，但此过程是否对神经干细胞/祖细胞增殖及分化产生影响呢？实验设计在体外单独或联合使用1，2-2.5和4-5倍临床血药浓度异丙酚和瑞芬太尼干预新生大鼠海马区神经干细胞/祖细胞。结果发现较低浓度的异丙酚和/或瑞芬太尼对细胞增殖和分化无明显影响，但中高浓度的异丙酚和/或瑞芬太尼能对神经干细胞/祖细胞增殖及分化产生显著的抑制作用，且高浓度瑞芬太尼和异丙酚单独或联合用药均会导致神经干细胞/祖细胞在分化初始期细胞内[Ca2+]i浓度显著下降。由此推测瑞芬太尼和异丙酚通过影响细胞内游离钙的浓度含量，进而干预神经干/祖细胞的增殖和分化。此结果是否可对临床麻醉用药剂量提出相关的实验性警示作用？

关键词: 神经再生, 异丙酚, 瑞芬太尼, 神经干细胞, 神经祖细胞, 增殖, 凋亡, 分化, [Ca2+]i

Abstract:

Propofol and remifentanil alter intracellular Ca2+ concentration ([Ca2+]i) in neural stem/progenitor cells by activating γ-aminobutyric acid type A receptors and by reducing testosterone levels. However, whether this process affects neural stem/progenitor cell proliferation and differentiation remains unknown. In the present study, we applied propofol and remifentanil, alone or in combination, at low, moderate or high concentrations (1, 2–2.5 and 4–5 times the clinically effective blood drug concentration), to neural stem/progenitor cells from the hippocampi of newborn rat pups. Low concentrations of propofol, remifentanil or both had no noticeable effect on cell proliferation or differentiation; however, moderate and high concentrations of propofol and/or remifentanil markedly suppressed neural stem/progenitor cell proliferation and differentiation, and induced a decrease in [Ca2+]i during the initial stage of neural stem/progenitor cell differentiation. We therefore propose that propofol and remifentanil interfere with the proliferation and differentiation of neural stem/progenitor cells by altering [Ca2+]i. Our findings suggest that propofol and/or remifentanil should be used with caution in pediatric anesthesia.

Key words: nerve regeneration, propofol, remifentanil, neural stem cells, neural progenitor cells, proliferation, apoptosis, differentiation, [Ca2+]i, neural regeneration