中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (4): 576-582.doi: 10.4103/1673-5374.155430

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

迷走神经电刺激保护脑缺血:与炎症有关的机制

  

  • 收稿日期:2015-03-24 出版日期:2015-04-22 发布日期:2015-04-22
  • 基金资助:

     北京市自然科学基金项目(7122164)

Electrical stimulation of the vagus nerve protects against cerebral ischemic injury through an anti-inflammatory mechanism

Yao-xian Xiang 1, Wen-xin Wang 1, Zhe Xue 1, Lei Zhu 2, Sheng-bao Wang 1, Zheng-hui Sun 1   

  1. 1 Department of Neurosurgery, Chinese PLA General Hospital, Beijing, China
    2 Department of Radiology, Beijing Electric Power Hospit, Beijng, China
  • Received:2015-03-24 Online:2015-04-22 Published:2015-04-22
  • Contact: Zheng-hui Sun, M.D., szh@sina.com.cn
  • Supported by:

    This study was supported by the Beijing Natural Science Foundation of China, No. 7122164.

摘要:

以线栓法建立局灶性脑缺血大鼠模型,造模后30min,12h,1d,2d,3d,7d和14d以电刺激右侧迷走神经(constant current of 0.5 mA, pulse width of 0.5 ms, and frequency of 20 Hz and continued 30 seconds every 5 minutes for a total period of 60 minutes)。结果发现经迷走神经刺激的脑缺血大鼠脑组织梗死体积明显缩小,神经功能明显改善,缺血脑组织中肿瘤坏死因子α和白细胞介素6表达明显下降。提示迷走神经刺激对脑缺血的保护作用可能与抑制肿瘤坏死因子α和白细胞介素6的表达有关。

关键词: 神经再生, 迷走神经刺激, 脑缺血, 炎症因子, 缺血体积, 神经功能

Abstract:

Vagus nerve stimulation exerts protective effects against ischemic brain injury; however, the underlying mechanisms remain unclear. In this study, a rat model of focal cerebral ischemia was established using the occlusion method, and the right vagus nerve was given electrical stimulation (constant current of 0.5 mA; pulse width, 0.5 ms; frequency, 20 Hz; duration, 30 seconds; every 5 minutes for a total of 60 minutes) 30 minutes, 12 hours, and 1, 2, 3, 7 and 14 days after surgery. Electrical stimulation of the vagus nerve substantially reduced infarct volume, improved neurological function, and decreased the expression levels of tumor necrosis factor-α and interleukin-6 in rats with focal cerebral ischemia. The experimental findings indicate that the neuroprotective effect of vagus nerve stimulation following cerebral ischemia may be associated with the inhibition of tumor necrosis factor-α and interleukin-6 expression.

Key words: nerve regeneration, vagus nerve stimulation, cerebral ischemia, inflammatory cytokines, infarct volume, neurological function, NSFC grants, neural regeneration