中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (6): 1011-1016.doi: 10.4103/1673-5374.300454

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

电针改善脑缺血再灌注大鼠学习记忆障碍与激活PI3K/Akt信号通路有关

  

  • 出版日期:2021-06-15 发布日期:2020-12-31
  • 基金资助:

    国家自然科学基金（81574042），河南省中医药专项研究项目（2018JDZX011）

Electroacupuncture improves learning and memory functions in a rat cerebral ischemia/reperfusion injury model through PI3K/Akt signaling pathway activation

Hui-Ling Wang1, Fei-Lai Liu1, Rui-Qing Li1, Ming-Yue Wan2, Jie-Ying Li3, Jing Shi3, Ming-Li Wu1, Jun-Hua Chen1, Wei-Juan Sun1, Hong-Xia Feng1, Wei Zhao1, Jin Huang3, Ren-Chao Liu3, Wen-Xue Hao2, Xiao-Dong Feng1, *   

  1. 1 Rehabilitation Center, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China;  2 Department of Rehabilitation Medicine, Fujian University of Chinese Medicine, Fuzhou, Fujian Province, China;  3 Department of Rehabilitation Medicine, Henan University of Chinese Medicine, Zhengzhou, Henan Province, China 
  • Online:2021-06-15 Published:2020-12-31
  • Contact: Xiao-Dong Feng, MD, PhD, fxd0502@163.com or 15136194032@163.com.
  • Supported by:
    The study was supported by the National Natural Science Foundation of China, No. 81574042, and the Traditional Chinese Medicine Special Research Projects of Henan Province of China, No. 2018JDZX011 (both to XDF).

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摘要：

电针已广泛用于治疗脑缺血后认知障碍，但其机制尚未完全阐明。研究显示自噬在认知障碍形成和发展中起重要作用，而PI3K/Akt信号通路在调节细胞自噬方面发挥重要的作用。为了解PI3K/Akt信号通路在电针治疗脑缺血再灌注大鼠模型中的作用，实验以线栓法建立大脑中动脉闭塞大鼠模型，造模2h后起连续8d以电针刺激百会和神庭（为膨胀波、频率1-20 Hz，峰值电压6 V，强度2 mA，每天30min/d）。（1）见电针刺激减小了模型大鼠的脑梗死体积，增加了缺血皮质中PI3K/Akt信号通路相关因子Beclin-1，mTOR和PI3K mRNA表达以及磷酸化Akt，Beclin-1，PI3K和mTOR蛋白的表达，同时减少了p53 mRNA和蛋白的表达；（2）在水迷宫测试中，电针刺激的大鼠导航测试潜伏期明显缩短，在空间探索测试穿越目标象限的次数增加；（3）见电针刺激百会和神庭穴后，激活了PI3K/Akt信号通路，从而出现了脑卒中后大鼠学习和记忆能力障碍得以改善的现象。动物实验于2016年3月10日在河南中医药大学附属第一医院伦理委员会批准，批准号：8150150901。

https://orcid.org/0000-0002-3806-7388 (Xiao-Dong Feng)

关键词:

中枢神经系统, 脑, 针刺, 卒中, 蛋白, 因子, 通路, 神经功能

Abstract: Electroacupuncture has been widely used to treat cognitive impairment after cerebral ischemia, but the underlying mechanism has not yet been fully elucidated. Studies have shown that autophagy plays an important role in the formation and development of cognitive impairment, and the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway plays an important role in autophagy regulation. To investigate the role played by the PI3K/Akt signaling pathway in the electroacupuncture treatment of cerebral ischemia/reperfusion rat models, we first established a rat model of cerebral ischemia/reperfusion through the occlusion of the middle cerebral artery using the suture method. Starting at 2 hours after modeling, electroacupuncture was delivered at the Shenting (GV24) and Baihui (GV20) acupoints, with a dilatational wave (1–20 Hz frequency, 2 mA intensity, 6 V peak voltage), for 30 minutes/day over 8 consecutive days. Our results showed that electroacupuncture reduced the infarct volume in a rat model of cerebral ischemia/reperfusion injury, increased the mRNA expression levels of the PI3K/Akt signaling pathway-related factors Beclin-1, mammalian target of rapamycin (mTOR), and PI3K, increased the protein expression levels of phosphorylated Akt, Beclin-1, PI3K, and mTOR in the ischemic cerebral cortex, and simultaneously reduced p53 mRNA and protein expression levels. In the Morris water maze test, the latency to find the hidden platform was significantly shortened among rats subjected to electroacupuncture stimulation compared with rats without electroacupuncture stimulation. In the spatial probe test, the number of times that a rat crossed the target quadrant was increased in rats subjected to electroacupuncture stimulation compared with rats without electroacupuncture stimulation. Electroacupuncture stimulation applied to the Shenting (GV24) and Baihui (GV20) acupoints activated the PI3K/Akt signaling pathway and improved rat learning and memory impairment. This study was approved by the Animal Ethics Committee of the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, China (approval No. 8150150901) on March 10, 2016. 

Key words: acupuncture, brain, central nervous system, factor, neurological function, pathways, protein, stroke