中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (8): 1258-1264.doi: 10.4103/1673-5374.162758

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

Notch信号通路的激活可促进脑创伤后神经血管的修复

  

  • 收稿日期:2015-07-08 出版日期:2015-08-24 发布日期:2015-08-24
  • 基金资助:

    贵州省自然科学基金(黔科合J字(2013)2311号)

Activation of the Notch signaling pathway promotes neurovascular repair after traumatic brain injury

Qi-shan Ran, Yun-hu Yu, Xiao-hong Fu, Yuan-chao Wen   

  1. Department of Neurosurgery, the First People’s Hospital of ZunYi/the Third Affiliated Hospital of ZunYi Medical College, Zunyi,
    Guizhou Province, China
  • Received:2015-07-08 Online:2015-08-24 Published:2015-08-24
  • Contact: Qi-shan Ran, ranqishan1203@126.com.
  • Supported by:

    This study was supported by the Natural Science Foundation of Guizhou Province in China, No. Qiankehe J (2013)2311.

摘要:

Notch信号通路是血管新生、内皮细胞形成的关键信号通路,但Notch信号通路是否参与内皮祖细胞对脑创伤后神经血管修复仍不清楚。因此,体外实验设计以转染质粒来特异性激活Notch信号通路,发现其可增强内皮祖细胞的迁移、侵袭和血管形成的能力;而通过siRNA特异性抑制Notch信号通路,内皮祖细胞迁移、侵袭和血管形成的能力受到抑制。对轻型脑创伤模型大鼠脑内穿刺注射JetPEITM系统(将Notch相关蛋白在脑创伤模型大鼠中过表达或敲低的系统)后,显示Notch信号通路激活促进了损伤脑组织神经血管修复能力。因此作者认为,激活Notch信号通路对脑创伤后神经血管的修复有确切的促进作用。

关键词: 神经再生, 内皮祖细胞, 创伤性颅脑损伤, Notch信号通路, 神经修复, 细胞迁移, 侵袭, 血管形成, JetPEITM系统

Abstract:

The Notch signaling pathway plays a key role in angiogenesis and endothelial cell formation, but it remains unclear whether it is involved in vascular repair by endothelial progenitor cells after traumatic brain injury. Therefore, in the present study, we controlled the Notch signaling pathway using overexpression and knockdown constructs. Activation of the Notch signaling pathway by Notch1 or Jagged1 overexpression enhanced the migration, invasiveness and angiogenic ability of endothelial progenitor cells. Suppression of the Notch signaling pathway with Notch1 or Jagged1 siRNAs reduced the migratory capacity, invasiveness and angiogenic ability of endothelial progenitor cells. Activation of the Notch signaling pathway in vivo in a rat model of mild traumatic brain injury promoted neurovascular repair. These findings suggest that the activation of the Notch signaling pathway promotes blood vessel formation and tissue repair after brain trauma.

Key words: