中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (12): 1976-1981.doi: 10.4103/1673-5374.172315

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

枸杞多糖促进成年大鼠视网膜前体细胞增殖

  

  • 收稿日期:2015-10-30 出版日期:2015-12-30 发布日期:2015-12-30

Lycium barbarum polysaccharides promotes in vivo proliferation of adult rat retinal progenitor cells

Hua Wang1, #, Benson Wui-Man Lau2, #, Ning-li Wang1, *, Si-ying Wang1, Qing-jun Lu1, Raymond Chuen-Chung Chang3, 5, 7, Kwok-fai So3, 4, 5, 6, 7, *   

  1. 1 Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing, China
    2 Department of Rehabilitation Science, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, China
    3 Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
    4 Department of Ophthalmology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
    5 The State Key Laboratory of Brain and Cognitive Sciences, The University of Hong Kong, Hong Kong Special Administrative Region, China
    6 GMH Institute of Central Nervous System Regeneration, Jinan University, Guangzhou, Guangdong Province, China
    7 Research Centre of Heart, Brain, Hormone and Healthy Aging, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
  • Received:2015-10-30 Online:2015-12-30 Published:2015-12-30
  • Contact: Kwok-fai So or Ning-li Wang,hrmaskf@hku.hk or wningli@trhos.com.

摘要:

枸杞是一味被广泛应用于保护视神经的中药,但其主要成分枸杞多糖对成年睫状体细胞增殖的影响尚不确定。为此,实验给予成年大鼠灌胃低、高剂量的枸杞多糖(1和10 mg/kg)共35d,并以灌胃PBS的大鼠为对照。结果发现,灌胃枸杞多糖大鼠睫状体中Ki-67标记细胞增殖数量明显高于PBS组,且尤以低剂量干预更为明显。双标染色结果显示大鼠视网膜睫状体Ki-67阳性细胞表达巢蛋白,但不表达神经胶质纤维酸性蛋白。说明枸杞多糖可促进成年睫状体中视网膜前体细胞的增殖,且细胞表现为神经元样表型。

关键词: 神经再生, 中医药, 枸杞多糖, 枸杞, 视网膜疾病, 睫状体, 神经发生, 成年

Abstract:

Lycium barbarum is a widely used Chinese herbal medicine prescription for protection of optic nerve. However, it remains unclear regarding the effects of Lycium barbarum polysaccharides, the main component of Lycium barbarum, on in vivo proliferation of adult ciliary body cells. In this study, adult rats were intragastrically administered low- and high-dose Lycium barbarum polysaccharides (1 and 10 mg/kg) for 35 days and those intragastrically administered phosphate buffered saline served as controls. The number of Ki-67-positive cells in rat ciliary body in the Lycium barbarum polysaccharides groups, in particular low-dose Lycium barbarum polysaccharides group, was significantly greater than that in the phosphate buffered saline group. Ki-67-positive rat ciliary body cells expressed nestin but they did not express glial fibrillary acidic protein. These findings suggest that Lycium barbarum polysaccharides can promote the proliferation of adult rat retinal progenitor cells and the proliferated cells present with neuronal phenotype.

Key words: nerve regeneration, traditional Chinese medicine, Lycium barbarum polysaccharides, Lycium barbarum (wolfberry), retinal disease, neurogenesis, adult, neural regeneration