中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (12): 1982-1988.doi: 10.4103/1673-5374.172316

• 原著:颅神经损伤修复保护与再生 • 上一篇    下一篇

外源性神经生长因子保护压榨伤舌下神经的作用

  

  • 收稿日期:2015-08-13 出版日期:2015-12-30 发布日期:2015-12-30

Exogenous nerve growth factor protects the hypoglossal nerve against crush injury

Li-yuan Fan1, 2, Zhong-chao Wang2, 3, Pin Wang1, 2, Yu-yan Lan1, 2, Ling Tu4, *   

  1. 1 Department of Prosthodontics, Stomatological Hospital of Sichuan Medical University, Luzhou, Sichuan Province, China
    2 Orofacial Reconstruction and Regeneration Laboratory, Sichuan Medical University, Luzhou, Sichuan Province, China
    3 Department of Endodontics, Stomatological Hospital of Sichuan Medical University, Luzhou, Sichuan Province, China
    4 Department of Anatomy and Physiology, College of Stomatology, Central South University, Changsha, Hunan Province, China
  • Received:2015-08-13 Online:2015-12-30 Published:2015-12-30
  • Contact: Ling Tu, M.D., xytl8466@qq.com.

摘要:

[摘要]研究表明感觉神经损伤可激活p38MAPK通路,但运动神经损伤后是否同样激活p38MAPK通路目前尚不清楚。一些研究已证实神经生长因子可在周围神经损伤后的修复过程中发挥作用,但未见对于舌下运动神经损伤修复的报道。我们设计了建立舌下神经压榨损伤大鼠模型,持续14d腹腔注射外源神经生长因子。发现舌下神经压榨损伤后受损神经元内p38MAPK活性增强,而外源性神经生长因子能发挥抑制作用,且使舌下神经核内运动神经元存活率增加。透射电镜证实注射神经生长因子后压榨损伤舌下神经形态有所恢复。说明外源性神经生长因子具有保护受损舌下神经元及促进神经再生的作用。

关键词: 神经再生, p38MAPK, MAPK, 神经生长因子, 舌下神经, 压榨损伤, 神经损伤

Abstract:

Studies have shown that sensory nerve damage can activate the p38 mitogen-activated protein kinase (MAPK) pathway, but whether the same type of nerve injury after exercise activates the p38MAPK pathway remains unclear. Several studies have demonstrated that nerve growth factor may play a role in the repair process after peripheral nerve injury, but there has been little research focusing on the hypoglossal nerve injury and repair. In this study, we designed and established rat models of hypoglossal nerve crush injury and gave intraperitoneal injections of exogenous nerve growth factor to rats for 14 days. p38MAPK activity in the damaged neurons was increased following hypoglossal nerve crush injury; exogenous nerve growth factor inhibited this increase in acitivity and increased the survival rate of motor neurons within the hypoglossal nucleus. Under transmission electron microscopy, we found that the injection of nerve growth factor contributed to the restoration of the morphology of hypoglossal nerve after crush injury. Our experimental findings indicate that exogenous nerve growth factor can protect damaged neurons and promote hypoglossal nerve regeneration following hypoglossal nerve crush injury.

Key words: nerve regeneration, p38MAPK, mitogen-activated protein kinase, nerve growth factor, hypoglossal nerve, crush injury, nerve injury, neural regeneration