中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (1): 132-136.doi: 10.4103/1673-5374.175059

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

局灶性脑缺血后NgR:两峰一低谷的时空表达

  

  • 收稿日期:2015-06-08 出版日期:2016-01-15 发布日期:2016-01-15
  • 基金资助:

    广东省科技项目(2013B022000098),广州医科大学2014年度科研项目(2014C26)

Spatiotemporal expression of Nogo-66 receptor after focal cerebral ischemia

Yue Cao 1, Ya-xian Dong 2, Jie Xu 3, Guo-liang Chu 3, Zhi-hua Yang 2, Yan-ming Liu 4   

  1. 1 Department of Medical Technology, Medical College of Shaoguan University, Shaoguan, Guangdong Province, China
    2 Department of Neurology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong Province, China
    3 Department of Human Anatomy, Sun Yat-Sen University, Guangzhou, Guangdong Province, China
    4 Department of Clinical Laboratory, Yue Bei People’s Hospital, Shaoguan, Guangdong Province, China
  • Received:2015-06-08 Online:2016-01-15 Published:2016-01-15
  • Contact: Jie Xu, xujie@gzsums.edu.cn.
  • Supported by:

    This study was supported by grants of the Guangdong Provincial Science and Technology Project in China, No. 2013B022000098 and the Scientific Research Project of Guangzhou Medical University in China in 2014, No. 2014C26.

摘要:

NgR是神经系统可塑性和再生干预的重要靶点。实验采用免疫组化方法检测大脑中动脉闭塞建立的局灶性脑缺血大鼠脑组织中NgR的表达变化。结果发现大鼠脑缺血中心区域不表达NgR,而边缘区中NgR表达明显增加;且缺血脑组织中NgR表达明显高于对侧,其中大脑皮质和海马(CA1,CA2和CA3区)中的NgR表达最多。另外大鼠脑组织中NgR表达在损伤后出现两峰一谷的表达特点,第1个峰值出现在1-3d,第2个峰值出现在28d,损伤后5d,NgR表达下降。

关键词: 局灶性脑缺血, 大脑皮质, 海马, NgR, Nogo-A, 免疫组化

Abstract:

NgR, the receptor for the neurite outgrowth inhibitor Nogo-66, plays a critical role in the plasticity and regeneration of the nervous system after injury such as ischemic stroke. In the present study, we used immunohistochemistry to investigate the regional expression of NgR in rat brain following middle cerebral artery occlusion (MCAO). NgR protein expression was not observed in the center of the lesion, but was elevated in the marginal zone compared with control and sham-operated rats. The cerebral cortex and hippocampus (CA1, CA2, and CA3) showed the greatest expression of NgR. Furthermore, NgR expression was higher in the ipsilesional hemisphere than on the control side in the same coronal section. Although time-dependent changes in NgR expression across brain regions had their own characteristics, the overall trend complied with the following rules: NgR expression changes with time showed two peaks and one trough; the first peak in expression appeared between 1 and 3 days after MCAO; expression declined at 5 days; and the second peak occurred at 28 days.

Key words: nerve regeneration, focal cerebral ischemia, cerebral cortex, hippocampus, NgR, Nogo-A, immunohistochemistry, neural regeneration