中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (1): 150-155.doi: 10.4103/1673-5374.175062

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

新型生物活性神经导管修复周围神经损伤

  

  • 收稿日期:2015-12-16 出版日期:2016-01-15 发布日期:2016-01-15
  • 基金资助:

    国家重点基础研究发展计划项目(2011CB606205),国家自然科学基金项目(51403168),教育部重大科技研究项目(313041),港澳台科技合作项目(2015DFH30180)

A novel bioactive nerve conduit for the repair of peripheral nerve injury

Bin-bin Li 1, 2, Yi-xia Yin 1, 2,Qiong-jiao Yan 1, 2, Xin-yu Wang 1, 2, Shi-pu Li 1, 2   

  1. 1 State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, Hubei Province, China
    2 Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan, Hubei Province, China
  • Received:2015-12-16 Online:2016-01-15 Published:2016-01-15
  • Contact: Yi-xia Yin,yinyixia@whut.edu.cn.
  • Supported by:

    This study was supported by a grant from the National Key Basic Research Program of China, No. 2011CB606205; a grant from the National Natural Science Foundation of China, No. 51403168; a grant from the Major Scientific and Technological Research Projects of the Ministry of Education of China, No. 313041; and a grant from the Scientific and Technological Cooperation Projects of Hong Kong, Macao and Taiwan, China, No. 2015DFH30180.

摘要:

神经导管的应用不仅可以避免自体移植的缺陷,而且也提供了一个调节周围神经再生中细胞因子、生长因子和神经营养因子的机会。实验利用聚(D,L-乳酸)构建神经修复导管,并以聚(乳酸-羟基乙酸-L-赖氨酸)和β-磷酸三钙加以修饰,以此导管修复大鼠10mm坐骨神经缺损。35d后以苏木精-伊红染色和甲苯胺蓝染色,发现与单纯聚(D,L-乳酸)构建的导管相比,新型导管修复后再生的神经纤维更粗更规整,且比目鱼肌形态更好。表明以聚乳酸为基础的生物活性神经修复导管修复损伤坐骨神经更具优势。

关键词: 神经再生, 聚乳酸, 聚(乳酸-羟基乙酸-L-赖氨酸), β-磷酸三钙, 神经导管, 神经纤维

Abstract:

The use of a nerve conduit provides an opportunity to regulate cytokines, growth factors and neurotrophins in peripheral nerve regeneration
and avoid autograft defects. We constructed a poly-D-L-lactide (PDLLA)-based nerve conduit that was modified using poly{(lactic acid)-co-[(glycolic acid)-alt-(L-lysine)]} and β-tricalcium phosphate. The effectiveness of this bioactive PDLLA-based nerve conduit was compared to that of PDLLA-only conduit in the nerve regeneration following a 10-mm sciatic nerve injury in rats. We observed the nerve morphology in the early period of regeneration, 35 days post injury, using hematoxylin-eosin and methylene blue staining. Compared with the PDLLA conduit, the nerve fibers in the PDLLA-based bioactive nerve conduit were thicker and more regular in size. Muscle fibers in the soleus muscle had greater diameters in the PDLLA bioactive group than in the PDLLA only group. The PDLLA-based bioactive nerve conduit is a promising strategy for repair after sciatic nerve injury.