中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (5): 929-935.doi: 10.4103/1673-5374.268926

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

右美托咪定和右酮洛芬对大鼠坐骨神经的传导阻滞

  

  • 出版日期:2020-05-15 发布日期:2020-06-01
  • 基金资助:
    土耳其科尼亚Selçuk大学科学委员会基金会支持

Effects of dexmedetomidine and dexketoprofen on the conduction block of rat sciatic nerve

Sengal Bagci Taylan1, Hulagu Bariskaner2   

  1. 1 Department of Medical Services and Techniques, Vocational School of Health Services, Hakkari University, Hakkari, Turkey
    2 Department of Pharmacology, School of Medicine, Selcuk University, Konya, Turkey
  • Online:2020-05-15 Published:2020-06-01
  • Contact: Sengal Bagci Taylan, PhD,sengalbagci08@gmail.com.
  • Supported by:
    This study was supported by Scientific Committee Foundation (No. 13102007) of Selçuk University, Konya, Turkey (to HB).

摘要:

右美托咪定是一种选择性α2-肾上腺素能受体激动剂,具有镇静、抗焦虑和镇痛作用,非选择性非甾体类抗炎药右酮洛芬常用于镇痛,右美托咪定和右酮洛芬作为周围神经局部麻醉剂的辅助用药在临床应用中逐渐增多。为了解不同剂量右美托咪定和右酮洛芬对大鼠坐骨神经的作用,实验将分离的成年大鼠坐骨神经置于包括灌注了新鲜Krebs 液的神经室及刺激和记录Ag-AgCl电极的系统中。在神经室中施用右美托咪定和右酮洛芬,累积浓度为10-9至10-5M,记录复合动作电位5和10min,计算相关参数。与未给药的对照组相比,右美托咪定和右酮洛芬均剂量依赖性抑制了复合动作电位参数。高剂量右美托咪定可抑制快传导神经纤维的传导,而右酮洛芬可时间依赖性地抑制慢传导神经纤维的传导。说明右美托咪定和右酮洛芬通过不同的作用方式使两者联合应用对周围神经起到较好的麻醉作用。

orcid: 0000-0002-8158-4487 (Sengal Bagci Taylan)

关键词: 右美托咪定, 右酮洛芬, 神经纤维, 复合动作电位, 坐骨神经, 大鼠, 神经室, 最大去极化

Abstract: Dexmedetomidine is a selective α2-adrenoceptor agonist that is used because of its sedative, anxiolytic, and analgesic effects. Dexketoprofen, which is used as an analgesic, is a nonselective nonsteroidal anti-inflammatory drug (NSAID). The use of dexmedetomidine and dexketoprofen as adjuvants to local anesthetics for the peripheral nerve is gradually increasing. In this study, we aimed to investigate the effects of different doses of dexmedetomidine and dexketoprofen on conduction block of rat sciatic nerve. The isolated sciatic nerve from adult rats was transferred to a nerve chamber. The compound action potentials (CAPs) were recorded from stimulated nerve with electrophysiological methods. Dexmedetomidine (n = 8) and dexketoprofen (n = 8) were administered in the chamber with cumulative concentrations of 10–9 to 10–5 M, and the CAPs were recorded for 5 and 10 minutes. The CAP parameters were calculated. Both dexmedetomidine and dexketoprofen significantly depressed all CAP parameters in a dose-dependent manner compared with the control group, i.e., the group in which rats did not receive treatment. CAP parameters showed there was no significant difference in nerve conduction inhibition between dexmedetomidine and dexketoprofen. Higher doses of dexmedetomidine suppressed the conduction in the fast-conducting fibers; however, dexketoprofen was found to suppress the conduction in the slow-conducting fibers in a time-dependent manner and suppress the conduction in the medium- and slow-conducting fibers in a dose-dependent manner. These findings suggest that dexmedetomidine and dexketoprofen exhibit better anesthetic effects on peripheral nerve through different ways of action. The experimental procedures were approved by the Necmettin Erbakan University on January 30, 2013 (approval No. 2013-024).

Key words: compound action potentials, dexketoprofen, dexmedetomidine, maximum depolarization, nerve chamber, nerve fibers, sciatic nerve