中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (5): 842-845.doi: 10.4103/1673-5374.177150

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

坐骨神经损伤后维生素B复合物与维生素B12水平的变化

  

  • 收稿日期:2015-12-22 出版日期:2016-05-20 发布日期:2016-05-20

Vitamin B complex and vitamin B12 levels after peripheral nerve injury

Idiris Altun1, *, Ergül Belge Kurutaş2   

  1. "1 Department of Neurosurgery, Kahramanmaras Sutcu Imam University Medical Faculty, Kahramanmaras, Turkey 2 Department of Biochemistry, Kahramanmaras Sutcu Imam University Medical Faculty, Kahramanmaras, Turkey"
  • Received:2015-12-22 Online:2016-05-20 Published:2016-05-20
  • Contact: Idiris Altun, M.D., idrisaltun46@hotmail.com.

摘要:

为评估大鼠模型周围神经挫伤组织中维生素B复合物与维生素B12水平是否改变。实验将80只大鼠随机分为未损伤对照组和坐骨神经挫伤后1,6,12,24h,3和7d 6个时间点组,分别给予其并应用ELISA法检测损伤部位维生素B复合物与维生素B12水平,发现相较于未损伤的对照组,维生素B复合物水平在损伤后1和12h较高,而在7d时较低;维生素B12水平则在损伤后1,6,12,24h明显降低。说明在周围神经损伤进程伴随着维生素B复合物与维生素B12水平的改变,也提示可以此作为周围神经修复的干预措施。 

orcid: 0000-0003-4263-766X(Idiris Altun)

关键词: 神经再生, 周围神经损伤, 坐骨神经损伤, 维生素B复合物, 维生素B12

Abstract:

"The aim of the present study was to evaluate whether tissue levels of vitamin B complex and vitamin B12 were altered after crush-induced peripheral nerve injury in an experimental rat model. A total of 80 male Wistar rats were randomized into one control (n = 8) and six study groups (1, 6, 12, 24 hours, 3, and 7 days after experimental nerve injury; n = 12 for each group). Crush-induced peripheral nerve injury was performed on the sciatic nerves of rats in six study groups. Tissue samples from the sites of peripheral nerve injury were obtained at 1, 6, 12, 24 hours, 3 and 7 days after experimental nerve injury. Enzyme-linked immunosorbent assay results showed that tissue levels of vitamin B complex and vitamin B12 in the injured sciatic nerve were significantly greater at 1 and 12 hours after experimental nerve injury, while they were significantly lower at 7 days than in control group. Tissue level of vitamin B12 in the injured sciatic nerve was significantly lower at 1, 6, 12 and 24 hours than in the control group. These results suggest that tissue levels of vitamin B complex and vitamin B12 vary with progression of crush-induced peripheral nerve injury, and supplementation of these vitamins in the acute period may be beneficial for acceleration of nerve regeneration."

Key words: nerve regeneration, sciatic nerve injury, vitamin B complex, vitamin B12, neural regeneration