中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (5): 745-751.doi: 10.4103/1673-5374.182700

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

布美他尼促进脑缺血慢性期海马齿状回神经前体细胞再生及树突发育

  

  • 收稿日期:2015-05-31 出版日期:2016-05-20 发布日期:2016-05-20

Bumetanide promotes neural precursor cell regeneration and dendritic development in the hippocampal dentate gyrus in the chronic stage of cerebral ischemia

Wang-shu Xu1, 2, #, Xuan Sun3, #, Cheng-guang Song1, 4, Xiao-peng Mu1, Wen-ping Ma5, Xing-hu Zhang2, Chuan-sheng Zhao1, *   

  1. "1 Department of Neurology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China 2 Neuroinfection and Neuroimmunology Center, Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China 3 Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China 4 Department of Neurology, Benxi Central Hospital of China Medical University, Benxi, Liaoning Province, China 5 Department of Medical Genetics, School of Basic Medicine, Peking University, Beijing, China"
  • Received:2015-05-31 Online:2016-05-20 Published:2016-05-20
  • Contact: Chuan-sheng Zhao, M.D., xws921@sina.com; cszhao@mail.cmu.edu.cn.

摘要:

布美他尼在脑缺血急性期应用能够减轻脑水肿及减少梗死面积,而对于脑缺血慢性期的神经保护及神经再生作用如何?研究较少。我们将内皮素1注入大鼠左侧大脑皮质运动区和左侧纹状体区制作脑缺血大鼠模型,7d后应用微量渗透泵连续21d侧脑室内注射布美他尼50μg/kg/d。发现注射布美他尼的脑缺血慢性期大鼠海马齿状回DCX阳性细胞数量及树突总长度增加,脑梗死体积缩小,水迷宫逃避潜伏期缩短并且穿越平台的次数明显增多。表明布美他尼能促进神经前体细胞的再生及树突发育,并且带来了促进大鼠认知功能恢复的效应,显示了其对缺血慢性期脑组织也具有良好的保护作用。

orcid: 0000-0002-4746-2064(Wang-shu Xu)

关键词: 神经再生, 脑缺血, 布美他尼, Na+-K+-2Cl- 同向转运蛋白1, 海马齿状回, 神经发生, 神经前体细胞, 树突发育, 认知功能恢复

Abstract:

"Bumetanide has been shown to lessen cerebral edema and reduce the infarct area in the acute stage of cerebral ischemia. Few studies focus on the effects of bumetanide on neuroprotection and neurogenesis in the chronic stage of cerebral ischemia. We established a rat model of cerebral ischemia by injecting endothelin-1 in the left cortical motor area and left corpus striatum. Seven days later, bumetanide 200 μg/kg/day was injected into the lateral ventricle for 21 consecutive days with a mini-osmotic pump. Results demonstrated that the number of neuroblasts cells and the total length of dendrites increased, escape latency reduced, and the number of platform crossings increased in the rat hippocampal dentate gyrus in the chronic stage of cerebral ischemia. These findings suggest that bumetanide promoted neural precursor cell regeneration, dendritic development and the recovery of cognitive function, and protected brain tissue in the chronic stage of ischemia."

Key words: nerve regeneration, cerebral ischemia, bumetanide, Na+-K+-2Cl&ndash, cotransporter 1, hippocampal dentate gyrus, neurogenesis, neural precursor cells, dendritic development, cognitive function, neural regeneration