中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (7): 1128-1133.doi: 10.4103/1673-5374.187049

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

Apelin-13可能是继发性损伤的新干预靶点

  

  • 出版日期:2016-07-30 发布日期:2016-07-30
  • 基金资助:
    中国国家自然科学基金项目（81302612）；江苏省自然科学基金项目（12KJB310018）；大学生创新培养项目（201410313023Z）

Apelin-13 as a novel target for intervention in secondary injury after traumatic brain injury

Hai-jun Bao1, #, Hai-yang Qiu1, #, Jin-xia Kuai2, Cheng-jie Song3, Shao-xian Wang1, Chao-qun Wang1, Hua-bin Peng1, Wen-can Han1, Yong-ping Wu1, *   

  1. 1 Department of Pathology, Xuzhou Medical College, Xuzhou, Jiangsu Province, China 2 Department of Forensic Medicine, Xuzhou Medical College, Xuzhou, Jiangsu Province, China 3 Department of Physiology, Xuzhou Medical College, Xuzhou, Jiangsu Province, China
  • Online:2016-07-30 Published:2016-07-30
  • Contact: Yong-ping Wu, wuyongping_123@tom.com.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 81302612; the Natural Science Foundation of Jiangsu Province of China, No. 12KJB310018; a grant from the Student Innovation Training Program of China, No. 201410313023Z.

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摘要：

研究显示，富集于神经系统中的脂肪因子Apelin-13可以通过抑制自噬而减轻脑缺血损伤。然而，Apelin-13是否也能对脑外伤继发性神经细胞凋亡和血脑屏障损伤产生影响，发挥脑保护作用，目前仍不明确。实验发现，脑外伤小鼠损伤侧（右侧）脑室注射Apelin-13，可使大脑含水量降低，血脑屏障破坏程度减轻，大脑皮质和海马中水通道蛋白4表达降低，皮质和海马中Caspase-3和Bax表达下降，细胞凋亡减少。实验结果表明Apelin-13可能是改善脑外伤后继发性损伤，产生神经保护作用的干预靶点。 

orcid: 0000-0002-9073-2429 (Yong-ping Wu)

关键词: 神经再生, 细胞凋亡, Apelin-13, 脑外伤, 脑水肿, 血脑屏障, 脑含水量, 水通道蛋白4, caspase-3

Abstract:

The adipocytokine, apelin-13, is an abundantly expressed peptide in the nervous system. Apelin-13 protects the brain against ischemia/ reperfusion injury and attenuates traumatic brain injury by suppressing autophagy. However, secondary apelin-13 effects on traumatic brain injury-induced neural cell death and blood-brain barrier integrity are still not clear. Here, we found that apelin-13 significantly decreases cerebral water content, mitigates blood-brain barrier destruction, reduces aquaporin-4 expression, diminishes caspase-3 and Bax expression in the cerebral cortex and hippocampus, and reduces apoptosis. These results show that apelin-13 attenuates secondary injury after traumatic brain injury and exerts a neuroprotective effect.

Key words: nerve regeneration, apoptosis, apelin-13, traumatic brain injury, brain edema, blood-brain barrier, brain water content, aquaporin-4, caspase-3, neural regeneration