中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (8): 1312-1321.doi: 10.4103/1673-5374.189197

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

枸杞多糖的抗氧化作用可促进损伤海绵体的神经修复与再生

  

  • 出版日期:2016-08-31 发布日期:2016-08-31
  • 基金资助:
    中国国家自然科学基金项目(81100492,81402119,81500517),山东省自然科学基金项目(ZR2014HP055,ZR2014HL071)

Antioxidative mechanism of Lycium barbarum polysaccharides promotes repair and regeneration following cavernous nerve injury

Zhan-kui Zhao1, 2, #, Hong-lian Yu3, #, Bo Liu1, Hui Wang1, Qiong Luo4, Xie-gang Ding1, *   

  1. 1 Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei Province, China 2 Department of Urology, Affliated Hospital of Jining Medical University, Jining, Shandong Province, China 3 Department of Medical Biochemistry, Basic Medical School, Jining Medical University, Jining, Shandong Province, China 4 Department of Nutrition and Food Health, School of Public Health, Wuhan University, Wuhan, Hubei Province, China
  • Online:2016-08-31 Published:2016-08-31
  • Contact: Xie-gang Ding, Ph.D., dingxiegang2000@126.com.
  • Supported by:
    This work was supported by grants from the National Natural Science Foundation of China, No. 81100492, 81402119 and 81500517; the natural Science Foundation of Shandong Province of China, No. ZR2014HP055 and ZR2014HL071.

摘要:

中药枸杞子的提取物枸杞多糖具抗氧化特性,可以减缓氧化应激对海绵状神经的损伤。为验证此假设,实验设计大鼠海绵体神经损伤后1,7,14d时给予为期2周的枸杞多糖灌胃。损伤后1d开始灌胃的大鼠在损伤后1和2周时血清超氧化物歧化酶和谷胱甘肽过氧化物酶活性均明显增加,损伤后2和4周时血清丙二醛水平下降;损伤后1d开始灌胃的大鼠在损伤后12周时海绵体内压峰值、海绵体神经有髓轴突数量、NADPH黄递酶阳性神经纤维数量、海绵体组织中内皮型一氧化氮合酶和3-硝基酪氨酸水平均高于损伤后7和14d开始灌胃的大鼠。结果表明,应用枸杞多糖灌胃干预海绵体神经损伤,可有效促进神经再生及勃起功能恢复,损伤后1d开始给药治疗效果最好。 

orcid: 0000-0001-9878-5210 (Xie-gang Ding)

关键词: 神经再生, 勃起功能障碍, 海绵体神经, 枸杞多糖, 氧化应激, 超氧化物歧化酶, 谷胱甘肽过氧化物酶, 丙二醛, 海绵体内压

Abstract: Polysaccharides extracted from Lycium barbarum exhibit antioxidant properties. We hypothesized that these polysaccharides resist oxidative stress-induced neuronal damage following cavernous nerve injury. In this study, rat models were intragastrically administered Lycium barbarum polysaccharides for 2 weeks at 1, 7, and 14 days after cavernous nerve injury. Serum superoxide dismutase and glutathione peroxidase activities signifcantly increased at 1 and 2 weeks post-injury. Serum malondialdehyde levels decreased at 2 and 4 weeks. At 12 weeks, peak intracavernous pressure, the number of myelinated axons and nicotinamide adenine dinucleotide phosphate-diaphorase-positive nerve fbers, levels of phospho-endothelial nitric oxide synthase protein and 3-nitrotyrosine were higher in rats administered at 1 day post-injury compared with rats administered at 7 and 14 days post-injury. These fndings suggest that application of Lycium barbarum polysaccharides following cavernous nerve crush injury effectively promotes nerve regeneration and erectile functional recovery. This neuroregenerative effect was most effective in rats orally administered Lycium barbarum polysaccharides at 1 day after cavernous nerve crush injury.

Key words: nerve regeneration, erectile dysfunction, cavernous nerve, Lycium barbarum polysaccharides, oxidative stress, superoxide dismutase; glutathione peroxidase, malondialdehyde, intracavernous pressure, neural regeneration