中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (11): 1797-1803.doi: 10.4103/1673-5374.194749

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

能减轻醋酸铅神经毒性的木蓝椭圆叶木姜子

  

  • 出版日期:2016-11-30 发布日期:2016-11-30

Neuroprotective potential of Indigofera oblongifolia leaf methanolic extract against lead acetate-induced neurotoxicity

Saleh Al-Quraishy1, Mohamed A. Dkhil1, 2, Shaimaa R. Ibrahim3, Ahmed E. Abdel Moneim2, *   

  1. 1 Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia 2 Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt 3 Department of Molecular Drug Evaluation, National Organization for Drug Control & Research (NODCAR), Giza, Egypt
  • Online:2016-11-30 Published:2016-11-30
  • Contact: Ahmed E. Abdel Moneim, aest1977@hotmail.com.

摘要:

药物植物木蓝椭圆叶木姜子已被证实有抗炎和抗肿瘤作用,但其叶子甲醇提取物对铅诱导的神经毒性的影响尚无报道,为此实验在大鼠腹腔注射醋酸铅(20mg/kg)前的1h内灌胃木蓝椭圆叶木姜子叶甲醇提取物(0.1g/kg)连续5d。结果发现,醋酸铅诱导了大鼠大脑脂质过氧化物和一氧化氮的增加,谷胱甘肽水平以及4种抗氧化酶超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶的降低;实时定量PCR还检测到醋酸铅诱导了大鼠大脑超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、Bcl-2 mRNA表达上调,而Bax mRNA表达下调;HE染色可观察到醋酸铅使大鼠大脑发生了明显的病理改变。木蓝椭圆叶木姜子叶甲醇提取物预处理可降低醋酸铅诱导的大鼠大脑过氧化水平、提高抗氧化酶活性和抗凋亡能力,减轻神经毒性。说明木蓝椭圆叶木姜子叶甲醇提取物通过抗氧化和抗凋亡,减轻醋酸铅诱导的神经毒性。

orcid: 0000-0002-2654-2591 (Ahmed E. Abdel Moneim)

关键词: 神经再生, 木蓝椭圆叶木姜子, 醋酸铅, 铅中毒, 神经毒性, 氧化应激, 凋亡

Abstract: Lead (Pb) is one of the most common environmental toxicants, exposure to which can cause signifcant neurotoxicity and an associated decline in brain function. Tis study investigated the possible neuroprotective role of Indigofera oblongifolia leaf methanolic extract (IOLME) against lead-induced neurotoxicity. Rats were intraperitoneally injected with lead acetate, with or without IOLME (intragastric administration for 5 days), and the neuroprotective effect of IOLME was assessed by measuring the lead concentration, redox status (lipid peroxidation, nitric oxide and glutathione), enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione peroxidase and reductase), PCR assays of apoptosis markers (Bax and Bcl-2) and histopathology of the brain. Te increases in the lipid peroxidation, nitric oxide, and apoptosis, the decreases in the glutathione level and the activity of antioxidant enzymes, and the altered histology of the brain induced by lead acetate were mitigated in the brain of rats pre-treated with IOLME. Tese fndings indicate that IOLME has benefcial effects and it mitigates lead acetate-induced neurotoxicity via its antioxidant and anti-apoptotic activities.

Key words: nerve regeneration, lead acetate, Indigofera oblongifolia, neurotoxicity, oxidative stress, apoptosis, neural regeneration