中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (11): 1830-1838.doi: 10.4103/1673-5374.194755

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

Rab27a/Slp2-a复合物影响许旺细胞髓鞘化

  

  • 出版日期:2016-11-30 发布日期:2016-11-30
  • 基金资助:
    中国国家自然科学基金项目(31071251, 81471255和81471259);国家重点基础研究发展计划(973计划)(2014CB542202);江苏省教育厅基础研究项目(14KJA310004);南通市科技局自然科学研究项目(HS2013014);南通大学自然科学研究项目(13Z008)

Rab27a/Slp2-a complex is involved in Schwann cell myelination

Wen-feng Su1, Yun Gu1, Zhong-ya Wei1, Yun-tian Shen1, Zi-han Jin1, Ying Yuan1, 2, Xiao-song Gu1, Gang Chen1, *   

  1. 1 Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China 2 Afliated Hospital of Nantong University, Nantong, Jiangsu Province, China
  • Online:2016-11-30 Published:2016-11-30
  • Contact: Gang Chen, Ph.D., M.D., chengang6626@ntu.edu.cn.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 31071251, 81471255, and 81471259; a grant from the Ministry of Science and Technology of China (973 Program), No. 2014CB542202; a grant from the Basic Research Program of Education Department of Jiangsu Province, China, No. 14KJA310004; a grant from the Natural Science Research Project of Nantong Science and Technology Bureau, China, No. HS2013014; and a grant from the Natural Science Research Project of Nantong University, China, No. 13Z008.

摘要:

周围神经系统中许旺细胞髓鞘化是一个涉及髓鞘蛋白转运的复杂的过程。最近研究发现,内涵体/溶酶体系统参与髓鞘的形成,我们既往研究结果表明一种小GTP酶Rab27a能调节许旺细胞中溶酶体胞吐作用和髓鞘蛋白的转运。实验建立了一个脊髓背根节神经元和许旺细胞共培养体系,以确定髓鞘形成过程中Rab27a 相关信号的作用。首先,Slp2-a在许旺细胞中内源性表达,为Rab27a的效应子;其次,许旺细胞髓鞘化过程中Rab27a表达显著上升;最后,沉默许旺细胞中Rab27a和Slp2-a不仅降低髓鞘蛋白的表达,而且还破坏共培养体系中髓鞘样膜的形成。因此实验结果说明Rab27a/Slp2-a复合物能在体外影响许旺细胞的髓鞘化过程。 

orcid: 0000-0003-3669-5687 (Gang Chen)

关键词: 神经再生, 许旺细胞, 背根节细胞, 共培养, 髓鞘蛋白, 髓鞘化, Rab27效应子, Rab27a, Slp2a

Abstract: Myelination of Schwann cells in the peripheral nervous system is an intricate process involving myelin protein trafcking. Recently, the role and mechanism of the endosomal/lysosomal system in myelin formation were emphasized. Our previous results demonstrated that a small GTPase Rab27a regulates lysosomal exocytosis and myelin protein trafcking in Schwann cells. In this present study, we established a dorsal root ganglion (DRG) neuron and Schwann cell co-culture model to identify the signals associated with Rab27a during myelination. First, Slp2-a, as the Rab27a effector, was endogenously expressed in Schwann cells. Second, Rab27a expression signifcantly increased during Schwann cell myelination. Finally, Rab27a and Slp2-a silencing in Schwann cells not only reduced myelin protein expression, but also impaired formation of myelin-like membranes in DRG neuron and Schwann cell co-cultures. Our fndings suggest that the Rab27a/ Slp2-a complex affects Schwann cell myelination in vitro.

Key words: nerve regeneration, Schwann cells, dorsal root ganglion neurons, co-culture, myelin proteins, myelination, Rab27 effectors, Rab27a, Slp2-a, neural regeneration