中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (12): 1962-1968.doi: 10.4103/1673-5374.197138

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

中药复方右归丸降低外源性糖皮质激素诱导的垂体前叶细胞凋亡

  

  • 收稿日期:2016-11-21 出版日期:2016-12-31 发布日期:2016-12-31
  • 基金资助:
    中国国家自然科学基金项目(30471576, 30872277)中国教育部大学创新研究项目(IRT0760)

Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells

Yong-zhi Ji1,*, Long Geng2, *, Hong-bo Zhou2, Hua-chen Wei3, Hong-duo Chen2   

  1. 1 Department of Dermatology, Second Hospital of Jilin University, Changchun, Jinlin Province, China 2 Department of Dermatology, First Hospital of China Medical University, Shenyang, Liaoning Province, China 3 Department of Dermatology, Mount Sinai Medical Center, New York, USA
  • Received:2016-11-21 Online:2016-12-31 Published:2016-12-31
  • Contact: Long Geng, Ph.D., M.D. or Yong-zhi Ji, Ph.D., cmugenglong@hotmail.com or jiyongzhi@126.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 30471576, and 30872277; and a grant from the Innovative Research Program in Universities of the Ministry of Education of China, No. IRT0760.

摘要:

长期应用糖皮质激素会抑制下丘脑-垂体-肾上腺轴分泌反应相关因子,而细胞凋亡参与糖皮质激素的副作用。中药复方制剂右归丸在临床上主要用来改善长期使用糖皮质激素引起的副作用,但其药理作用机制尚不明朗。我们假设右归丸对地塞米松干预的垂体前叶细胞有抗凋亡作用,通过体内实验发现,右归丸有效降低了地塞米松干预大鼠垂体前叶细胞凋亡的数量,促凋亡蛋白蛋白细胞色素c、caspase-3和caspase-9免疫反应和mRNA表达,提升了抗凋亡因子Bcl-2免疫反应和mRNA表达。体外实验表明,右归丸含药血清有效减少地塞米松引起的大鼠垂体前叶细胞中线粒体膜电位下降细胞及凋亡细胞的数量,实验结果可说明右归丸通过线粒体介导的凋亡通路减少了糖皮质激素诱导大鼠垂体前叶细胞的凋亡。 

orcid: 0000-0002-4754-4967 (Long Geng), 0000-0002-0696-2142 (Yong-zhi Ji)

关键词: 神经再生, 右归丸, 糖皮质激素, 细胞凋亡, bcl-2, 细胞色素c, caspase-3, caspase-9, 线粒体膜电位

Abstract: Long-term glucocorticoid use may result in sustained suppression of one or more secreted components from the hypothalamo-pituitary-adrenal axis, and ofen results in apoptosis. Yougui Pill (YGP), a 10-component traditional Chinese herbal medicine, has been shown to be clinically effective for glucocorticoid-induced suppression of the hypothalamo-pituitary-adrenal axis. However, the pharmacological and molecular mechanisms remain unclear. We hypothesized that YGP would exert an anti-apoptosis effect on dexamethasone-treated anterior pituitary cells. In vivo experiments showed that YGP signifcantly reduced the number of apoptotic cells, down-regulated mRNA expression of cytochrome c, caspase-3, and caspase-9, and up-regulated mRNA expression of Bcl-2. Tese fndings suggest that YGP reduced glucocorticoid-induced apoptosis in rat anterior pituitary cells by regulating the mitochondria-mediated apoptosis pathway.

Key words: nerve regeneration, Yougui Pill, glucocorticoid, apoptosis, Bcl-2, cytochrome c, caspase-3, caspase-9, mitochondrial membrane potential, neural regeneration