中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (12): 2018-2024.doi: 10.4103/1673-5374.197147

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

“三法三穴”调节坐骨神经损伤大鼠脊髓p38MAPK的表达

  

  • 收稿日期:2016-11-12 出版日期:2016-12-31 发布日期:2016-12-31
  • 基金资助:
    中国国家自然科学基金项目(81373759);北京市自然科学基金项目(7142097)

“Tree Methods and Tree Points” regulates p38 mitogen-activated protein kinase in the dorsal horn of the spinal cord in a rat model of sciatic nerve injury

Xin Guo1, Tian-yuan Yu1, *, Wong Steven1, Wen-duan Jia1, Chi Ma1, Yan-hong Tao1, Chao Yang1, Tao-tao Lv1, Shuai Wu1, Meng-qian Lu1, Jia-li Liu2   

  1. 1 College of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, China 2 College of Taditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China
  • Received:2016-11-12 Online:2016-12-31 Published:2016-12-31
  • Contact: Tian-yuan Yu, Ph.D., yutianyuan@sina.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81373759; the Natural Science Foundation of Beijing of China, No. 7142097.

摘要:

推拿方法是一种治疗由外周神经损伤引起的感觉障碍及相关疾病的中国中医药传统治疗方法之一。作者既往研究发现,点、拨、揉法对殷门(BL 37)、委中(BL 40)、阳陵泉(GB 34),即“三法三穴”推拿,可以改善坐骨神经损伤大鼠的行为学指标,防止肌肉萎缩,保护脊髓神经元,从而促进坐骨神经的修复,但其机制尚不明确。维持,本次实验通过挤压建立大鼠坐骨神经损伤模型,然后以“三法三穴”法进行推拿,1次/d,连续20d。结果显示了这种推拿方法可以降低坐骨神经损伤大鼠的热痛阈值,恢复腓肠肌湿重,促进坐骨神经形态的恢复,同时降低脊髓背角中磷酸化p38MAPK、肿瘤坏死因子α和白细胞介素1β的水平。说明“三法三穴” 通过调控脊髓背角磷酸化p38MAPK,肿瘤坏死因子α以及白细胞介素1β的表达,促进周围神经损伤动物形态学的恢复以及行为学的改善。 

orcid: 0000-0002-5455-5833 (Xin Guo), 0000-0003-2089-3101 (Tian-yuan Yu)

关键词: 神经再生, 推拿, “三法三穴”, 磷酸化p38MAPK, 坐骨神经损伤, 肿瘤坏死因子α, 白细胞介素1β, 脊髓背角

Abstract: Tuina is a traditional Chinese treatment for sensory disturbances caused by peripheral nerve injury and related diseases. Our previous studies showed that tuina regulates relevant regions and indices of the spinal dorsal horn using the Dian, Bo, and Rou method in Yinmen (BL37), Yanglingquan (GB34), and Weizhong (BL40). Treatment prevents muscle atrophy, protects spinal cord neurons, and promotes sciatic nerve repair. Te mechanisms of action of tuina for treating peripheral nerve injury remain poorly understood. Tis study established rat models of sciatic nerve injury using the crushing method. Rats received Chinese tuina in accordance with the principle of “Tree Methods and Tree Points,” once daily for 20 days. Tuina intervention reduced paw withdrawal latency and improved wet weight of the gastrocnemius muscle, as well as promoting morphological recovery of sciatic nerve fbers, Schwann cells, and axons. Te protein expression levels of phospho-p38 mitogen-activated protein kinase, tumor necrosis factor-α, and interleukin-1β also decreased. Tese fndings indicate that “Tree Methods and Tree Points” promoted morphological recovery and improved behavior of rats with peripheral nerve injury

Key words: nerve regeneration, tuina, Tree Methods and Tree Points, phospho-p38 mitogen-activated protein kinase, sciatic nerve injury, tumor necrosis factor-α, interleukin-1β, dorsal horn of the spinal cord, neural regeneration