中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (12): 2025-2030.doi: 10.4103/1673-5374.197148

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

周围神经损伤后神经细胞粘附分子L1和信号素3A的表达

  

  • 收稿日期:2016-06-16 出版日期:2016-12-31 发布日期:2016-12-31
  • 基金资助:
    国家自然科学基金项目（81371389，31500927，31300942，81201017），江苏省高校基础研究计划资助项目（13KJB180018），南通大学自然科学项目（14ZY013）

Expression changes of nerve cell adhesion molecules L1 and semaphorin 3A afer peripheral nerve injury

Qian-ru He1, #, Meng Cong1, #, Qing-zhong Chen2, Ya-feng Sheng1, Jian Li2, Qi Zhang1, Fei Ding1, Yan-pei Gong2, *   

  1. 1 Jiangsu Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China 2 Afliated Hospital of Nantong University, Nantong, Jiangsu Province, China
  • Received:2016-06-16 Online:2016-12-31 Published:2016-12-31
  • Contact: Yan-pei Gong, M.D., gonghand@hotmail.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81371389, 31500927, 31300942, 81201017; the Collegiate Natural Science Foundation of Jiangsu Province of China, No. 13KJB180018; the Natural Science Foundation of Nantong University of China, No. 14ZY013.

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摘要：

神经细胞粘附分子L1在中枢神经系统神经元生长锥的表达与轴突的引导方向密切相关，而其在周围神经趋化性再生中究竟发挥怎样的作用目前尚不清楚。为此，本次实验希望能够揭示这一问题。(1)qPCR结果显示损伤2周时感觉神经近侧端L1表达高于运动神经，而神经导向因子信号素3A则相反。(2)免疫印迹结果显示，在损伤后L1先在运动神经近侧端表达高于感觉神经，2周后感觉神经中表达较高；信号素3A在损伤后3d和1周时在运动神经中表达高于运动神经。在神经远侧端L1和信号素3A在损伤后3d、1周和2周时在运动神经中高于感觉神经。(3)免疫组化结果也显示,神经损伤后2周L1在感觉神经近侧端的表达高于运动神经，而信号素3A在运动神经远侧端的表达高于感觉神经。 (4)提示L1和信号素3A在感觉和运动神经损伤后的近侧端和远侧端呈现不同的表达模式,在神经趋化性再生过程中发挥着重要作用。 

orcid: 0000-0002-4847-9669 (Yan-pei Gong)

关键词: 神经再生, 神经细胞粘附分子L1, 信号素3A, 感觉神经, 运动神经, 神经损伤, 趋化性再生

Abstract: The expression of nerve cell adhesion molecule L1 in the neuronal growth cone of the central nervous system is strongly associated with the direction of growth of the axon, but its role in the regeneration of the peripheral nerve is still unknown. Tis study explored the problem in a femoral nerve section model in rats. L1 and semaphorin 3A mRNA and protein expressions were measured over the 4-week recovery period. Quantitative polymerase chain reaction showed that nerve cell adhesion molecule L1 expression was higher in the sensory nerves than in motor nerves at 2 weeks afer injury, but vice versa for the expression of semaphorin 3A. Western blot assay results demonstrated that nerve cell adhesion molecule L1 expression was higher in motor nerves than in the sensory nerves at the proximal end afer injury, but its expression was greater in the sensory nerves at 2 weeks. Semaphorin 3A expression was higher in the motor nerves than in the sensory nerves at 3 days and 1 week afer injury. Nerve cell adhesion molecule L1 and semaphorin 3A expressions at the distal end were higher in the motor nerves than in the sensory nerves at 3 days, 1 and 2 weeks. Immunohistochemical staining results showed that nerve cell adhesion molecule L1 expression at the proximal end was greater in the sensory nerves than in the motor nerves; semaphorin 3A expression was higher in the motor nerves than in the sensory nerves at 2 weeks afer injury. Taken together, these results indicated that nerve cell adhesion molecules L1 and semaphorin 3A exhibited different expression patterns at the proximal and distal ends of sensory and motor nerves, and play a coordinating role in neural chemotaxis regeneration.

Key words: nerve regeneration, neural cell adhesion molecule L1, semaphorin 3A, sensory nerve, motor nerve, peripheral nerve injury, chemotaxis regeneration, neural regeneration