中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (11): 1843-1852.doi: 10.4103/1673-5374.219045

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

新生大鼠大脑发育过程中NG2和血小板衍化生长因子α受体表达的变化

  

  • 收稿日期:2017-10-12 出版日期:2017-11-15 发布日期:2017-11-15
  • 基金资助:

    中国国家自然科学基金项目（31100769）

Expression of NG2 and platelet-derived growth factor receptor alpha in the developing neonatal rat brain

Ping Li1, 2, Heng-xi Li2, Hong-yan Jiang2, Lie Zhu3, Hai-ying Wu4, Jin-tao Li5, Jiang-hua Lai1   

  1. 1 College of Forensic Science, Health Science Center, Xi’an Jiaotong University, Xi’an, Shaanxi Province, China
    2 Department of Anatomy and Histology & Embryology, Faculty of Basic Medical Sciences, Kunming Medical University, Kunming, Yunnan Province, China
    3 Department of Plastic Surgery, Changzheng Hospital, Shanghai, China
    4 Department of Emergency and Intensive Care Unit, First Affiliated Hospital, Kunming Medical University, Kunming, Yunnan Province, China
    5 Neuroscience Institute, Faculty of Basic Medical Sciences, Kunming Medical University, Kunming, Yunnan Province, China
  • Received:2017-10-12 Online:2017-11-15 Published:2017-11-15
  • Contact: Jiang-hua Lai, Ph.D.,laijh1011@xjtu.edu.cn.
  • Supported by:

    This study was supported by grants from the National Natural Science Foundation of China, No. 31100769.

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摘要：

既往研究认为血小板衍化生长因子α受体是中枢神经系统少突胶质前体细胞的标记物。随着NG2的发现，随后研究认为NG2也是少突胶质前体细胞的标记物。但是血小板衍化生长因子α受体和NG2作为新生大鼠大脑发育过程中少突胶质前体细胞的标记物是否存在差异，目前尚不可知。实验以免疫组化染色分析出生后3-14d的大鼠大脑，见NG2阳性细胞在大脑皮质外锥体细胞层、内锥体细胞层和多形细胞层，以及胼胝体、外囊、梨状皮质和中隔内侧核中存在；NG2阳性细胞呈星状或梭形，存在较长的突起，随着大脑发育突起逐渐减少并缩短；血小板衍化生长因子α受体阳性细胞的分布及形态与NG2阳性细胞一致；免疫荧光双标染色可确认NG2和血小板衍化生长因子α受体在胞体和突起的共定位，且NG2和血小板衍化生长因子α受体双阳性细胞主要在出生后早期可见，在出生后3-14d时NG2和血小板衍化生长因子α受体双阳性细胞以及血小板衍化生长因子α受体阳性细胞数量减少，而NG2阳性细胞数量增加。实验还发现正常新生大脑巨噬细胞之一的胼胝体阿米巴样小胶质细胞不表达NG2，但经缺氧诱导后则表达NG2，而正常或缺氧条件下均未发现血小板衍化生长因子α受体。结果说明NG2与血小板衍化生长因子α受体一样可以有效标示少突胶质前体细胞，且NG2还能参与胼胝体阿米巴样小胶质细胞的炎性病理过程。

orcid:0000-0003-0851-4412(Jiang-hua Lai)

关键词: 神经再生, NG2, 血小板衍化生长因子α受体, 少突胶质前体细胞, 阿米巴样小胶质细胞, OX-42, 缺氧, 大脑皮质, 胼胝体

Abstract:

Platelet-derived growth factor receptor alpha (PDGFRα) is a marker of oligodendrocyte precursor cells in the central nervous system. NG2 is also considered a marker of oligodendrocyte precursor cells. However, whether there are differences in the distribution and morphology of oligodendrocyte precursor cells labeled by NG2 or PDGFRα in the developing neonatal rat brain remains unclear. In this study,by immunohistochemical staining, NG2 positive (NG2+) cells were ubiquitous in the molecular layer, external pyramidal layer, internal pyramidal layer, and polymorphic layer of the cerebral cortex, and corpus callosum, external capsule, piriform cortex, and medial septal nucleus. NG2+ cells were stellate or fusiform in shape with long processes that were progressively decreased and shortened over the course of brain development. The distribution and morphology of PDGFRα positive (PDGFRα+) cells were coincident with NG2+ cells. The colocalization of NG2 and PDGFRα in the cell bodies and processes of some cells was confirmed by double immunofluorescence labeling.Moreover, cells double-labeled for NG2 and PDGFRα were predominantly in the early postnatal stage of development. The numbers of NG2+/PDGFRα+ cells and PDGFRα+ cells decreased, but the number of NG2+ cells increased from postnatal days 3 to 14 in the developing brain. In addition, amoeboid microglial cells of the corpus callosum, newborn brain macrophages in the normal developing brain, did not express NG2 or PDGFRα, but NG2 expression was detected in amoeboid microglia after hypoxia. The present results suggest that NG2 and PDGFRα are specific markers of oligodendrocyte precursor cells at different stages during early development. Additionally, the NG2 protein is involved in inflammatory and pathological processes of amoeboid microglial cells.

Key words: nerve regeneration, NG2, platelet-derived growth factor receptor alpha, oligodendrocyte precursor cells, amoeboid microglial cells, OX-42, hypoxia, cerebral cortex, corpus callosum, neural regeneration