中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (5): 877-886.doi: 10.4103/1673-5374.232485

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

脊髓损伤犬的扩散张量成像参数与神经功能评分的动态相关性

  

  • 收稿日期:2018-04-03 出版日期:2018-05-15 发布日期:2018-05-15
  • 基金资助:

    中国国家自然科学基金项目（81272164），中央公共科研机构基础科学研究专项基金（2015CZ-6, 2016CZ-4），北京脑病研究所项目（201601,0000-100031），十二五计划项目（2012BAI34B02），科技部项目（2015CB351701）

Dynamic correlation of diffusion tensor imaging and neurological function scores in beagles with spinal cord injury

 Chang-Bin Liu1, De-Gang Yang1, Qian-Ru Meng1, Da-Peng Li1, Ming-Liang Yang1, Wei Sun1, Wen-Hao Zhang1, Chang Cai1, Liang-Jie Du1, Jun Li1, Feng Gao1, Yan Yu1, Xin Zhang1, Zhen-Tao Zuo2, Jian-Jun Li1   

  1. 1 School of Rehabilitation Medicine, Capital Medical University; Department of Spinal and Neural Function Reconstruction, China Rehabilitation Research Center; Center of Neural Injury and Repair, Beijing Institute for Brain Disorders; China Rehabilitation Science Institute; Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China;
    2 State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China; The Innovation Center of Excellence on Brain Science, Chinese Academy of Sciences; Sino-Danish College, University of Chinese Academy of Sciences, Beijing, China
  • Received:2018-04-03 Online:2018-05-15 Published:2018-05-15
  • Contact: Jian-Jun Li,13718331416@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81272164; the Special Fund for Basic Scientific Research of Central Public Research Institutes in China, No. 2015CZ-6,2016CZ-4; the Beijing Institute for Brain Disorders in China, No. 201601,0000-100031; the Supporting Program of the “Twelve Five-year Plan” for Science & Technology Research of China, No.2012BAI34B02; a grant from the Ministry of Science and Technology of China, No. 2015CB351701.

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摘要：

对于损伤后的脊髓结构与功能的关系至关重要。定量扩散张量成像可以提供有关神经组织微观结构的信息，具有量化脊髓白质和灰质病理损伤的潜力。为此，实验建立了以改良的脊髓挫伤撞击器撞击损伤比格犬T10段脊髓，然后在脊髓损伤后3，24h，6和12周时以扩散张量成像观察脊髓整体、白质与灰质结构的变化，以德克萨斯脊髓损伤评分评价其后肢神经功能的变化。见随着脊髓损伤时间的延长脊髓损伤扩散张量成像中分数各向异性呈下降趋势，而表观扩散系数、平均扩散系数和径向扩散系数呈先下降后上升的趋势，整个脊髓表观扩散系数与德克萨斯脊髓损伤评分高度相关（R = 0.919，P = 0.027）；白质表观扩散系数与德克萨斯脊髓损伤评分高度相关（R = 0.932，P = 0.021），分数各向异性与德克萨斯脊髓损伤评分中度相关（R = 0.549，P = 0.338）；灰质表观扩散系数与德克萨斯脊髓损伤评分高度相关（R = 0.882，P = 0.048）。实验数据结果说明，白质表观扩散系数与神经功能存在最为显著的相关性，可用于预测脊髓损伤后神经功能恢复的情况。

orcid:0000-0003-2663-5971(Jian-Jun Li)

关键词: 脊髓损伤, 扩散张量成像, 分数各向异性, 表观扩散系数, 白质, 灰质, 神经行为, 德克萨斯脊髓损伤评分, 犬

Abstract:

Exploring the relationship between different structure of the spinal cord and functional assessment after spinal cord injury is important.Quantitative diffusion tensor imaging can provide information about the microstructure of nerve tissue and can quantify the pathological damage of spinal cord white matter and gray matter. In this study, a custom-designed spinal cord contusion-impactor was used to damage the T10 spinal cord of beagles. Diffusion tensor imaging was used to observe changes in the whole spinal cord, white matter, and gray matter, and the Texas Spinal Cord Injury Score was used to assess changes in neurological function at 3 hours, 24 hours, 6 weeks,and 12 weeks after injury. With time, fractional anisotropy values after spinal cord injury showed a downward trend, and the apparent diffusion coefficient, mean diffusivity, and radial diffusivity first decreased and then increased. The apparent diffusion-coefficient value was highly associated with the Texas Spinal Cord Injury Score for the whole spinal cord (R = 0.919, P = 0.027), white matter (R = 0.932, P = 0.021), and gray matter (R = 0.882, P = 0.048). Additionally, the other parameters had almost no correlation with the score (P > 0.05).In conclusion, the highest and most significant correlation between diffusion parameters and neurological function was the apparent diffusion-coefficient value for white matter, indicating that it could be used to predict the recovery of neurological function accurately after spinal cord injury.

Key words: nerve regeneration, spinal cord injury, diffusion tensor imaging, fractional anisotropy, apparent diffusion coefficient, white matter, gray matter, Texas Spinal Cord Injury Score, beagles, neural regeneration