中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (6): 1007-1012.doi: 10.4103/1673-5374.233443

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

手术脑损伤后植入胶原质黏多糖支架：基质金属蛋白酶2和9表达增强伴随神经发生

  

  • 收稿日期:2018-04-02 出版日期:2018-06-15 发布日期:2018-06-15
  • 基金资助:

    本研究得到中国国家科学理事会（NSC 102-2314-B-303-004）和慈济医疗基金会慈济医疗任务项目105-06的资助

Enhancement of matrix metalloproteinases 2 and 9 accompanied with neurogenesis following collagen glycosaminoglycan matrix implantation after surgical brain injury

Wei-Cherng Hsu1, 2, Chun-Hsien Yu2, 3, Woon-Man Kung4, 5, Kuo-Feng Huang2, 4   

  1. 1 Department of Ophthalmology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan, China;
    2 School of Medicine, Buddhist Tzu Chi University, Hualien, Taiwan, China;
    3 Department of Pediatrics, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan, China;
    4 Division of Neurosurgery, Department of Surgery, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan, China;
    5 Department of Exercise and Health Promotion, College of Education, Chinese Culture University, Taipei, Taiwan, China
  • Received:2018-04-02 Online:2018-06-15 Published:2018-06-15
  • Contact: Kuo-Feng Huang, M.D., Ph.D.,kuofeng1234@gmail.com.
  • Supported by:

    This study was supported by grants from the National Science Council of China (NSC 102-2314-B-303-004) and the Tzu Chi Medical Mission Project 105-06, Buddhist Tzu Chi Medical Foundation

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摘要：

手术脑损伤可导致不可逆的神经缺陷。我们以往的研究显示，植入胶原质黏多糖（CGM）支架可实现创伤性损伤脑组织的部分再生。基质金属蛋白酶（MMPs）可能在神经发生中起重要作用。实验旨在进一步研究手术脑损伤后胶原糖胺多糖支架植入后基质金属蛋白酶和神经发生之间的关系。实验将大鼠随机分为3组：假手术（仅限开颅手术），脑损伤组，脑损伤+胶原质黏多糖组（建立手术创伤致脑损伤大鼠模型组后，植入基质金属蛋白酶支架）。分别通过免疫荧光和ELISA法检测病灶边界区的SOX2阳性细胞（增殖性神经祖细胞标志物）和基质金属蛋白酶（MMP2和MMP9）水平。结果显示，手术脑创伤模型植入胶原质黏多糖支架后，病灶边界区（LBZ）内可见基质金属蛋白酶2 +/SOX2 +细胞和基质金属蛋白酶9 +/SOX2 +细胞数量明显增加；基质金属蛋白酶2和基质金属蛋白酶9水平也增加。这些发现表明，在手术脑外伤后单独植入胶原质黏多糖支架可以增强伴有神经发生的基质金属蛋白酶2和9的表达。

orcid:0000-0001-8242-1690(Kuo-Feng Huang)

 

关键词: 胶原糖胺聚糖, 基质金属蛋白酶, 手术脑外伤, 神经发生, 神经再生

Abstract:

Surgical brain injury may result in irreversible neurological deficits. Our previous report showed that partial regeneration of a traumatic brain lesion is achieved by implantation of collagen glycosaminoglycan (CGM). Matrix metalloproteinases (MMPs) may play an important role in neurogenesis but there is currently a lack of studies displaying the relationship between the stimulation of MMPs and neurogenesis after collagen glycosaminoglycan implantation following surgical brain trauma. The present study was carried out to further examine the expression of MMP2 and MMP9 after implantation of collagen glycosaminoglycan (CGM) following surgical brain trauma. Using the animal model of surgically induced brain lesion,we implanted CGM into the surgical trauma. Rats were thus divided into three groups: (1) sham operation group: craniotomy only; (2) lesion (L) group: craniotomy + surgical trauma lesion; (3) lesion + CGM (L +CGM) group: CGM implanted following craniotomy and surgical trauma lesion. Cells positive for SOX2 (marker of proliferating neural progenitor cells) and matrix metalloproteinases (MMP2 and MMP9) in the lesion boundary zone were assayed and analyzed by immunofluorescence and ELISA commercial kits,respectively. Our results demonstrated that following implantation of CGM after surgical brain trauma,significant increases in MMP2+/SOX2+ cells and MMP9+/SOX2+ cells were seen within the lesion boundary zone in the L + CGM group. Tissue protein concentrations of MMP2 and MMP9 also increased after CGM scaffold implantation. These findings suggest that implantation of a CGM scaffold alone after surgical brain trauma can enhance the expression of MMP2 and MMP9 accompanied by neurogenesis.

Key words: collagen glycosaminoglycan, matrix metalloproteinases, surgical brain trauma, neurogenesis, neural regeneration