中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (7): 1263-1268.doi: 10.4103/1673-5374.235074

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

葛根素可改善周围神经损伤大鼠异常性疼痛和痛觉过敏

  

  • 收稿日期:2018-05-25 出版日期:2018-07-15 发布日期:2018-07-15
  • 基金资助:

    国家自然科学基金(81671891)

Puerarin ameliorates allodynia and hyperalgesia in rats with peripheral nerve injury

Heng-Tao Xie, Zhong-Yuan Xia*, Xia Pan, Bo Zhao, Zhi-Gang Liu   

  1. Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China
  • Received:2018-05-25 Online:2018-07-15 Published:2018-07-15
  • Contact: Zhong-Yuan Xia, M.D.,heng_823@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81671891.

摘要:

葛根素是一种中国传统中药葛根的有效成分,常用于治疗心肌缺血和脑缺血,也有报道称葛根素具有镇痛作用,但葛根素对周围神经病理性疼痛的镇痛作用及作用机制少见报道。为此,实验采用坐骨神经部分结扎术制备神经病理性疼痛模型大鼠,每隔24 h腹腔注射葛根素30或60 mg/kg连续干预7 d。见神经病理性痛模型大鼠在造模后1天即出现明显的机械刺激痛和热敏痛。葛根素干预的神经病理性疼痛大鼠的机械/热痛阈值均显著增加,于造模后第7天最为明显,呈剂量依赖性。造模后7 d,实时荧光定量PCR检测结果显示,葛根素可明显逆转神经病理性疼痛大鼠背根神经节瞬时受体电位香草酸亚型1 (Trpv1)和色氨酸蛋白1 (Trpa1)通道的mRNA表达的增高,呈剂量依赖性。上述结果说明,葛根素可剂量依赖性地改善外周神经损伤导致的模型大鼠机械刺激痛和热敏痛,该过程可能通过抑制神经病理性疼痛模型大鼠背根神经节内Trpv1和Trpa1基因表达的上调而实现。

orcid:0000-0002-4292-6579(Zhong-Yuan Xia)

关键词: 葛根素, 葛根, 中药, Trpv1, Trpa1, 背根神经节, 周围神经损伤, 神经病理性疼痛, 机械性疼痛, 热痛过敏, 周围神经损伤, 神经再生

Abstract:

Puerarin is a major active ingredient of the traditional Chinese plant medicine, Radix Puerariae, and commonly used in the treatment of myocardial and cerebral ischemia. However, the effects of puerarin on neuropathic pain are still unclear. In this study, a neuropathic pain animal model was created by partial sciatic nerve ligation. Puerarin (30 or 60 mg/kg) was intraperitoneally injected once a day for 7 days.Mechanical allodynia and thermal hyperalgesia were examined at 1 day after model establishment. Mechanical threshold and paw withdrawal latency markedly increased in a dose-dependent manner in puerarin-treated rats, especially at 7 days after model establishment.At 7 days after model establishment, quantitative real-time reverse transcriptase-polymerase chain reaction results showed that puerarin administration reversed mRNA expression of transient receptor potential vanilloid 1 (Trpv1) and transient receptor potential ankyrin 1 (Trpa1) in a dose-dependent manner in dorsal root ganglion neurons after peripheral nerve injury. These results suggest that puerarin dose-dependently ameliorates neuropathic pain by suppressing Trpv1 and Trpa1 up-regulation in dorsal root ganglion of neuropathic pain rats.

Key words: nerve regeneration, puerarin, Radix Puerariae, traditional Chinese medicine, Trpv1, Trpa1, dorsal root ganglion, peripheral nerve injury, neuropathic pain, mechanical allodynia, thermal hyperalgesia, neural regeneration