中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (8): 1417-1424.doi: 10.4103/1673-5374.235297

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

血府逐瘀汤增强突触蛋白表达改善创伤性脑损伤后神经功能障碍

  

  • 收稿日期:2018-04-08 出版日期:2018-08-15 发布日期:2018-08-15
  • 基金资助:

    中国国家自然科学基金项目（81673719, 81173175, 81303074），中国博士后科学基金项目（2016M600639, 2017T100614）

Xuefu Zhuyu decoction improves neurological dysfunction by increasing synapsin expression after traumatic brain injury

Lin Zhu1, Tao Tang1, Rong Fan1, Jie-Kun Luo1, Han-Jin Cui1, Chun-Hu Zhang1, Wei-Jun Peng2, Peng Sun3, Xin-Gui Xiong1, Yang Wang1   

  1. 1 Laboratory of Ethnopharmacology, Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, Hunan Province, China;
    2 Department of Integrated Traditional Chinese and Western Medicine, Second Xiangya Hospital, Central South University, Changsha, Hunan Province, China;
    3 College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, China
  • Received:2018-04-08 Online:2018-08-15 Published:2018-08-15
  • Contact: Yang Wang, Ph.D., M.D.,wangyang_xy87@csu.edu.cn.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 81673719, 81173175 and 81303074; a grant from China Postdoctoral Science Foundation, No. 2016M600639 and 2017T100614.

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摘要：

中药组方血府逐瘀汤虽在临床上用于治疗创伤性脑损伤，改善脑外伤后功能障碍，但其作用机制尚待进一步研究。实验拟通过控制性脑皮质撞击法建立创伤性脑损伤模型大鼠，然后每天一次灌胃9，18 g/kg血府逐瘀汤至损伤后14/21d。以改良神经功能评分及Morries水迷宫评价模型大鼠神经功能的变化，以免疫组化、western blot以及定量实时PCR分析模型大鼠脑损伤区突触蛋白及对应mRNA的表达变化。结果显示，血府逐瘀汤能明显改善创伤性脑损伤模型大鼠的神经功能，且这些变化伴随着损伤侧脑组织中突触素、突触蛋白I和突触后密度蛋白95蛋白及mRNA表达的增加，呈剂量依赖性。提示血府逐瘀汤可提高突触蛋白表达，从而改善创伤性脑损伤后神经障碍。

orcid:0000-0003-0447-3107(Yang Wang)

 

关键词: 神经再生, 创伤性脑损伤, 血府逐瘀汤, 神经功能障碍, 突触蛋白, 突触素, 突触蛋白I, PSD-95, 神经保护

Abstract:

Xuefu Zhuyu decoction has been used for treating traumatic brain injury and improving post-traumatic dysfunction, but its mechanism of action needs further investigation. This study established rat models of traumatic brain injury by controlled cortical impact. Rat models were intragastrically administered 9 and 18 g/kg Xuefu Zhuyu decoction once a day for 14 or 21 days. Changes in neurological function were assessed by modified neurological severity scores and the Morris water maze. Immunohistochemistry, western blot assay, and reverse-transcription polymerase chain reaction were used to analyze synapsin protein and mRNA expression at the injury site of rats. Our results showed that Xuefu Zhuyu decoction visibly improved neurological function of rats with traumatic brain injury. These changes were accompanied by increased expression of synaptophysin, synapsin I, and postsynaptic density protein-95 protein and mRNA in a dose-dependent manner. These findings indicate that Xuefu Zhuyu decoction increases synapsin expression and improves neurological deficits after traumatic brain injury.

Key words: nerve regeneration, traumatic brain injury, Xuefu Zhuyu decoction, neurological dysfunction, synapsin, synaptophysin, synapsin I, postsynaptic density protein-95, neuroprotection, neural regeneration