中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (9): 1602-1608.doi: 10.4103/1673-5374.237123

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

百会透曲鬓针刺可抑制脑出血区域的细胞凋亡

  

  • 收稿日期:2018-05-28 出版日期:2018-09-15 发布日期:2018-09-15
  • 基金资助:

    中国国家自然科学基金项目（30772840，81273824，81473764）

Baihui (DU20)-penetrating-Qubin (GB7) acupuncture inhibits apoptosis in the perihemorrhagic penumbra

 Beng Zhang1, 2, Xiao-Hong Dai2, Xue-Ping Yu2, Wei Zou2, 3, Wei Teng2, Xiao-Wei Sun2, Wei-Wei Yu2, Hao Liu4, Hui Wang1, Meng-Juan Sun1, Meng Li2   

  1. 1 Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China
    2 First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang Province, China
    3 Clinical Key Laboratory of Integrated Traditional Chinese and Western Medicine, Heilongjiang University of Chinese Medicine, Harbin,Heilongjiang Province, China
    4 Department of Acupuncture and Moxibustion, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang Province, China
  • Received:2018-05-28 Online:2018-09-15 Published:2018-09-15
  • Contact: Wei Zou,kuangzou1965@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 30772840, 81273824, and 81473764.

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摘要：

有研究认为百会透曲鬓针刺可抑制脑出血后炎症反应和激活与增殖有关的信号通路。然而，目前还没有任何研究表明这种治疗与细胞凋亡之间的关系。实验设计在大鼠右侧尾状核中注射60μL自体血建立脑出血模型，6h后开始给予针刺刺激，针从百会进入，从曲鬓透出，手动交替沿顺时针和逆时针方向捻转，180±10转/min，6 min/次，刺激3次/d，间隔6min，连续治疗7 d。同时连续7 d每天1 mg/kg sonic hedgehog通路激活剂purmorphamine腹腔注射治疗的脑出血模型大鼠作为阳性对照。以Ludmila Belayev测试评估2组模型大鼠运动和感觉功能，以苏木精-伊红染色观察出血半暗带组织的病理损伤，以TUNEL染色检测出血半暗带中细胞的凋亡，以Western blot分析出血半暗带中SMO和Gli1的表达。发现百会透曲鬓针刺治疗可明显改善脑出血模型大鼠的运动和感觉功能，减少出血半暗带中的病理损伤及细胞凋亡，上调出血半暗带中SMO和Gli1的表达，表明百会透曲鬓针刺可通过刺激Sonic Hedgehog通路，减轻脑出血损伤，并促进其恢复。

orcid:0000-0003-4560-1582(Wei Zou)

 

关键词: 针刺治疗, 脑出血, 神经功能, Ludmila Belayev测试, TUNEL染色, Purmorphamine, SMO, PTCH1, 神经再生

Abstract:

Baihui (DU20)-penetrating-Qubin (GB7) acupuncture can inhibit inflammatory reactions and activate signaling pathways related to proliferation after intracerebral hemorrhage. However, there is no research showing the relationship between this treatment and cell apoptosis. Rat models of intracerebral hemorrhage were established by injecting 60 μL of autologous blood into the right side of the caudate-putamen. Six hours later, the needle traveled subcutaneously from the Baihui acupoint to Qubin acupoint. The needle was alternately rotated (180 ± 10 turns/min) manually along clockwise and counter-clockwise directions. Stimulation lasted for 7 days, and was performed three times each for 6 minutes with 6-minute intervals between stimulations. Rats intraperitoneally receiving Sonic hedgehog pathway activator, purmorphamine (1 mg/kg per day), served as positive controls. Motor and sensory function were assessed using the Ludmila Belayev test. Extent of pathological changes were measured in the perihemorrhagic penumbra using hematoxylin-eosin staining. Apoptosis was examined by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling assay. Expression of smoothened (Smo) and glioma-associated homolog 1 (Gli1) was determined by western blot assay. Our results showed that Baihui-penetrating-Qubin acupuncture promoted recovery of motor and sensory function, reduced the apoptotic cell percentage in the perihemorrhagic penumbra, and up-regulated Smo and Gli1 expression. We conclude that Baihui-penetrating-Qubin acupuncture can mitigate hemorrhage and promote functional recovery of the brain in a rat model of intracerebral hemorrhage, possibly by activating the Sonic hedgehog pathway.

Key words: nerve regeneration, acupuncture treatment, intracerebral hemorrhage, neurological function, Ludmila Belayev test, TUNEL assay, purmorphamine, Smo, Ptch1, Glil, neural regeneration