中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (10): 1804-1810.doi: 10.4103/1673-5374.237127

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

周围神经损伤后黏着连接途径相关基因的转录组学分析

  

  • 收稿日期:2018-06-20 出版日期:2018-10-15 发布日期:2018-10-15
  • 基金资助:

    国家自然科学基金（31700926）；江苏省高等教育机构重点学科建设项目

Transcriptome analysis of adherens junction pathway-related genes after peripheral nerve injury

Sheng Yi, Xing-Hui Wang, Ling-Yan Xing   

  1. Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
  • Received:2018-06-20 Online:2018-10-15 Published:2018-10-15
  • Contact: Ling-Yan Xing, PhD,xlyan@ntu.edu.cn.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 31700926; the Priority Academic Program Development of Jiangsu Higher Education Institutions of China.

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摘要：

周围神经系统的再生过程是由广泛的分子和途径驱动的，其中黏着连接对细胞间的连接和周围神经组织的完整性是至关重要的。然而，很少有研究系统地描述周围神经损伤后黏着连接途径的转录变化。实验采用钳压法建立坐骨神经损伤大鼠模型，对损伤后0，1，4，7和14 d的深度测序数据进行了全面的转录组学分析，发现大鼠坐骨神经损伤后，多数黏着连接及黏着连接重塑过程中的相关基因可出现表达上调或下调的动态变化。实验也采用RT-PCR检测对黏着连接和黏着连接相关重塑信号通路中关键基因ARPC1B，ARPC3，TUBA8，TUBA1C，CTNNA2，ACTN3，MET，HGF，NME1和ARF6在损伤后1，4，7和14 d的表达量进行了定量分析，验证发现除CNTTA2基因表达下调外，这些关键基因都在损伤后表达上调。这些数据揭示了黏着连接和黏着连接重塑过程信号通路中关键基因在周围神经损伤后呈动态表达的变化，这些关键基因可为周围神经损伤临床治疗靶点选择提供参考基础。

orcid:0000-0002-7646-2522(Ling-Yan Xing)

 

关键词: 周围神经损伤, 坐骨神经夹伤, RNA测序, 黏着连接, 黏着连接重塑, 韦恩图, IPA通路分析, 差异表达基因, 热火图, 全面转录分析, 转录组学, 神经再生

Abstract:

The neural regeneration process is driven by a wide range of molecules and pathways. Adherens junctions are critical cellular junctions for the integrity of peripheral nerves. However, few studies have systematically characterized the transcript changes in the adherens junction pathway following injury. In this study, a rat model of sciatic nerve crush injury was established by forceps. Deep sequencing data were analyzed using comprehensive transcriptome analysis at 0, 1, 4, 7, and 14 days after injury. Results showed that most individual molecules in the adherens junctions were either upregulated or downregulated after nerve injury. The mRNA expression of ARPC1B, ARPC3, TUBA8, TUBA1C, CTNNA2, ACTN3, MET, HGF, NME1 and ARF6, which are involved in the adherens junction pathway and in remodeling of adherens junctions, was analyzed using quantitative real-time polymerase chain reaction. Most of these genes were upregulated in the sciatic nerve stump following peripheral nerve injury, except for CTNNA2, which was downregulated. Our findings reveal the dynamic changes of key molecules in adherens junctions and in remodeling of adherens junctions. These key genes provide a reference for the selection of clinical therapeutic targets for peripheral nerve injury.

Key words: peripheral nerve regeneration, crushed sciatic nerve, RNA-seq, adherens junctions, remodeling of adherens junctions, Venn diagram, ingenuity pathway analysis, differentially expressed genes, comprehensive transcript analysis, transcriptomics, heatmap, neural regeneration