中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (9): 1651-1656.doi: 10.4103/1673-5374.255996

• 原著:周围神经损伤修复保护与再生 • 上一篇    

新型miRNA——miR-sc14可促进许旺细胞的增殖和迁移

  

  • 出版日期:2019-09-15 发布日期:2019-09-15
  • 基金资助:

    江苏省高等学校优先学科项目

Novel miRNA, miR-sc14, promotes Schwann cell proliferation and migration

Xi-Meng Ji 1, 2 , Shan-Shan Wang 1, 3 , Xiao-Dong Cai 1 , Xing-Hui Wang 1 , Qian-Yan Liu 1 , Pan Wang 1 , Zhang-Chun Cheng 1 , Tian-Mei Qian 1   

  1. 1 Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
    2 Nonnasality Clinical Medicine, Nanjing Medical University, Nanjing, Jiangsu Province, China
    3 Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China
  • Online:2019-09-15 Published:2019-09-15
  • Contact: Tian-Mei Qian, MS, qiantm86@ntu.edu.cn.
  • Supported by:

    This study was supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions of China.

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摘要：

miRNA是一类内源性短链非编码RNA，可介导多种生物学功能。miRNA可调节周围神经的各种生理和病理活动，其中包括神经修复和再生。作者所在团队曾利用大鼠坐骨神经损伤模型发现了许多新的miRNA，其中包括miR-sc14。此次实验以实时RT-PCR检测坐骨神经缺损大鼠神经残端中miR-sc14的表达变化，发现其在坐骨神经损伤后发生显著改变，o损伤上1d开始增加，而7d时下降。然后EdU细胞增殖实验和Tranwell细胞迁移实验结果表明，转染miR-sc14模拟物可以促进许旺细胞的增殖和迁移，而转染miR-sc14抑制剂可以抑制许旺细胞的增殖和迁移。另外，生物信息学分析研究了miR-sc14的潜在靶基因，发现成纤维细胞生长因子受体2）可能是miR-sc14的潜在靶基因。实验结果揭示了神经损伤后不同时间点大鼠坐骨神经中miR-sc14的表达变化，发现上调的miR-sc14可促进许旺细胞的增殖和迁移，因而miR-sc14可能成为促进周围神经的损伤修复的干预靶点。

orcid: 0000-0002-2179-1206 (Tian-Mei Qian)

关键词: 新型miRNA, miR-sc14, 周围神经损伤, 细胞增殖, 细胞迁移, 许旺细胞, 成纤维细胞生长因子受体2, 生物功能, 周围神经再生, 调控机制, 神经再生

Abstract:

MicroRNAs refer to a class of endogenous, short non-coding RNAs that mediate numerous biological functions. MicroRNAs regulate var¬ious physiological and pathological activities of peripheral nerves, including peripheral nerve repair and regeneration. Previously, using a rat sciatic nerve injury model, we identified many functionally annotated novel microRNAs, including miR-sc14. Here, we used real-time reverse transcription-polymerase chain reaction to examine miR-sc14 expression in rat sciatic nerve stumps. Our results show that miR-sc14 is noticeably altered following sciatic nerve injury, being up-regulated at 1 day and diminished at 7 days. EdU and transwell chamber assay results showed that miR-sc14 mimic promoted proliferation and migration of Schwann cells, while miR-sc14 inhibitor suppressed their proliferation and migration. Additionally, bioinformatic analysis examined potential target genes of miR-sc14, and found that fibro¬blast growth factor receptor 2 might be a potential target gene. Specifically, our results show changes of miR-sc14 expression in the sciatic nerve of rats at different time points after nerve injury. Appropriately, up-regulation of miR-sc14 promoted proliferation and migration of Schwann cells. Consequently, miR-sc14 may be an intervention target to promote repair of peripheral nerve injury. The study was ap¬proved by the Jiangsu Provincial Laboratory Animal Management Committee, China on March 4, 2015 (approval No. 20150304-004).

Key words: nerve regeneration, novel microRNAs, miR-sc14, peripheral nerve injury, cell proliferation, cell migration, Schwann cells, fibroblast growth factor receptor 2, biological functions, peripheral nerve regeneration, regulatory mechanisms, neural regeneration