中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (2): 375-381.doi: 10.4103/1673-5374.346461

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

富血小板血浆可有效促进损伤坐骨神经后周围神经的再生

  

  • 出版日期:2023-02-15 发布日期:2022-08-08
  • 基金资助:
    吉林省科技厅项目（3D5195941430，20190201087）；吉林省财政厅项目（3D517DV93429）

Platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury

Su-Long Wang1, #, Xi-Lin Liu1, #, Zhi-Chen Kang2, Yue-Shu Wang1, *   

  1. 1Department of Hand Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China; 2Department of Rehabilitation, The Second Hospital of Jilin University, Changchun, Jilin Province, China
  • Online:2023-02-15 Published:2022-08-08
  • Contact: Yue-Shu Wang, PhD, yueshu@jlu.edu.cn.
  • Supported by:
    This study was supported by grants from the Department of Technology of Jilin Province, Nos. 3D5195941430 (to YSW), 20190201087 (to ZCK), and the Department of Finance of Jilin Province, No. 3D517DV93429 (to ZCK).

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摘要：

富血小板血浆对神经再生的作用效果仍存在争议。因此实验建立了保留外膜的坐骨神经小间隙缺损兔模型，然后以富血小板血浆填充缺损区域进行修复，结果显示，4.5-6.5倍以及7.5-8.5倍全血血小板浓度的富血小板对神经损伤有着较好的修复效果。进而以蛋白质组学分析富血小板血浆促进神经再生的可能机制，结果发现，富血小板血浆可促进神经损伤后参与血管生成的整合素β8的表达，差异表达蛋白主要富集于粘着斑通路。以蛋白质相互作用网络分析筛选出的2种关键蛋白RSP27a和UBQLN1可参与调节体内泛素水平。由此推测，富血小板血浆可能通过影响血管生成以及泛素水平促进坐骨神经损伤后周围神经的再生。

https://orcid.org/0000-0001-6484-9319 (Yue-Shu Wang)

关键词: 周围神经损伤, 神经再生, 富血小板血浆, 富白细胞-血小板血浆, 蛋白质组学, 坐骨神经损伤, 生物信息学分析, 兔, 整合素β8

Abstract: The effect of platelet-rich plasma on nerve regeneration remains controversial. In this study, we established a rabbit model of sciatic nerve small-gap defects with preserved epineurium and then filled the gaps with platelet-rich plasma. Twenty-eight rabbits were divided into the following groups (7 rabbits/group): model, low-concentration PRP (2.5–3.5-fold concentration of whole blood platelets), medium-concentration PRP (4.5–6.5-fold concentration of whole blood platelets), and high-concentration PRP (7.5–8.5-fold concentration of whole blood platelets). Electrophysiological and histomorphometrical assessments and proteomics analysis were used to evaluate regeneration of the sciatic nerve. Our results showed that platelet-rich plasma containing 4.5–6.5- and 7.5–8.5-fold concentrations of whole blood platelets promoted repair of sciatic nerve injury. Proteomics analysis was performed to investigate the possible mechanism by which platelet-rich plasma promoted nerve regeneration. Proteomics analysis showed that after sciatic nerve injury, platelet-rich plasma increased the expression of integrin subunit β-8 (ITGB8), which participates in angiogenesis, and differentially expressed proteins were mainly enriched in focal adhesion pathways. Additionally, two key proteins, ribosomal protein S27a (RSP27a) and ubiquilin 1 (UBQLN1), which were selected after protein-protein interaction analysis, are involved in the regulation of ubiquitin levels in vivo. These data suggest that platelet-rich plasma promotes peripheral nerve regeneration after sciatic nerve injury by affecting angiogenesis and intracellular ubiquitin levels. 

Key words: bioinformatic analysis, ITGB8, leukocyte-platelet rich plasma, nerve regeneration, peripheral nerve injury, platelet-rich plasma, proteomic analysis, sciatic nerve injury