中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (6): 1364-1370.doi: 10.4103/1673-5374.354517

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

胶质源性神经营养因子和脑源性神经营养因子参与调节星形胶质细胞-许旺细胞的相互作用

  

  • 出版日期:2023-06-15 发布日期:2023-01-05
  • 基金资助:
    中国国家自然科学基金项目(82171213),福建省自然科学基金项目(2019J01289)

Glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor regulate the interaction between astrocytes and Schwann cells at the trigeminal root entry zone

Madeha Ishag Adam1, #, Ling Lin2, #, Amir Mahmoud Makin3, Xiao-Fen Zhang1, Lu-Xi Zhou1, Xin-Yue Liao1, Li Zhao1, Feng Wang1, *, Dao-Shu Luo1, *   

  1. 1Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian Province, Laboratory of Clinical Applied Anatomy, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province, China; 2Public Technology Service Center of Fujian Medical University, Fuzhou, Fujian Province, China; 3Center for Membrane and Water Science & Technology, Institute of Oceanic and Environmental Chemical Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang Province, China
  • Online:2023-06-15 Published:2023-01-05
  • Contact: Dao-Shu Luo, PhD, luods2004@fjmu.edu.cn; Feng Wang, PhD, fjwf95168@163.com.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China in 2021, No. 82171213; and the Natural Science Foundation of Fujian Province in 2019, No. 2019J01289 (both to DSL).

摘要:

三叉神经根区是中枢性少突胶质细胞髓鞘和周围性许旺细胞髓鞘的交界区,其特殊的解剖生理结构决定了三叉神经根区具有神经损伤易感性。临床上大部分原发性三叉神经痛的病因与三叉神经根区微血管压迫密切相关。为开发一种模拟三叉神经根区神经胶质环境的有效体外模型,作为研究胶质源性神经营养因子和脑源性神经营养因子对三叉神经根区结构和功能完整性影响以及参与调节细胞相互作用的工具,实验从大鼠三叉神经根区中分离的原代星形胶质细胞和许旺细胞,使用两孔硅培养插件接种星形胶质细胞和许旺细胞来模拟三叉神经根区微环境,并以胶质源性神经营养因子和脑源性神经营养因子进行干预。结果发现,单独培养条件下胶质源性神经营养因子即对许旺细胞有明显促迁移作用,但对星形胶质细胞的迁移影响不明显。共培养体系中,胶质源性神经营养因子可促进星形胶质细胞与许旺细胞的双向迁移。脑源性神经营养因子可显著促进星形胶质细胞的活化和迁移,但在共培养体系中脑源性神经营养因子对星形胶质细胞和许旺细胞的迁移则有一定的抑制作用。表明胶质源性神经营养因子和脑源性神经营养因子在三叉神经根区共培养系统中参与调节星形胶质细胞-许旺细胞的相互作用,这可作为细胞模型以研究与三叉神经损伤相关的胶质细胞失调机制以及可能的治疗干预措施。

https://orcid.org/0000-0001-9013-1052 (Dao-Shu Luo); 

https://orcid.org/0000-0002-8637-6660 (Feng Wang); 

https://orcid.org/0000-0001-7819-3998 (Amir Mahmoud Makin)

关键词: 脑源性神经营养因子, 胶质源性神经营养因子, 三叉神经, 星形胶质细胞, 许旺细胞, 细胞迁移, 胶质细胞相互作用

Abstract: The trigeminal root entry zone is the zone at which the myelination switches from peripheral Schwann cells to central oligodendrocytes. Its special anatomical and physiological structure renders it susceptible to nerve injury. The etiology of most primary trigeminal neuralgia is closely related to microvascular compression of the trigeminal root entry zone. This study aimed to develop an efficient in vitro model mimicking the glial environment of trigeminal root entry zone as a tool to investigate the effects of glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor on the structural and functional integrity of trigeminal root entry zone and modulation of cellular interactions. Primary astrocytes and Schwann cells isolated from trigeminal root entry zone of postnatal rats were inoculated into a two-well silicon culture insert to mimic the trigeminal root entry zone microenvironment and treated with glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor. In monoculture, glial cell line-derived neurotrophic factor promoted the migration of Schwann cells, but it did not have effects on the migration of astrocytes. In the co-culture system, glial cell line-derived neurotrophic factor promoted the bidirectional migration of astrocytes and Schwann cells. Brain-derived neurotrophic factor markedly promoted the activation and migration of astrocytes. However, in the co-culture system, brain-derived neurotrophic factor inhibited the migration of astrocytes and Schwann cells to a certain degree. These findings suggest that glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor are involved in the regulation of the astrocyte-Schwann cell interaction in the co-culture system derived from the trigeminal root entry zone. This system can be used as a cell model to study the mechanism of glial dysregulation associated with trigeminal nerve injury and possible therapeutic interventions. 

Key words: astrocytes, brain-derived neurotrophic factor, cell migration, glial cell line-derived neurotrophic factor, glial interaction, Schwann cells, trigeminal nerve