中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (5): 1062-1066.doi: 10.4103/1673-5374.355768

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

双靶区神经环路磁刺激减弱脊髓损伤后星形胶质细胞活化以改善运动功能 

  

  • 出版日期:2023-05-15 发布日期:2022-11-01
  • 基金资助:
    中国国家自然科学基金项目(81772453,81974358),上海市重点临床专业计划项目(shslczdzk02701)

Double-target neural circuit-magnetic stimulation improves motor function in spinal cord injury by attenuating astrocyte activation

Dan Zhao1, 2, #, Ye Zhang3, #, Ya Zheng2, Xu-Tong Li4, Cheng-Cheng Sun2, Qi Yang2, Qing Xie1, *, Dong-Sheng Xu5, 6, 7, *   

  1. 1Department of Rehabilitation Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China;  2Department of Rehabilitation, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China;  3Department of Rehabilitation, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China;  4Department of Neurology, Zibo Centre Hospital, Zibo, Shandong Province, China;  5Department of Rehabilitation Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, China;  6School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China;  7Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation, Shanghai, China
  • Online:2023-05-15 Published:2022-11-01
  • Contact: Qing Xie, MS, ruijin_xq@163.com; Dong-Sheng Xu, PhD, dxu0927@shutcm.edu.cn.
  • Supported by:
    The study was supported by the National Natural Science Foundation of China, Nos. 81772453 and 81974358 (both to DSX) and Shanghai Municipal Key Clinical Specialty Program, No. shslczdzk02701 (to QX).

摘要:

基于神经环路调控概念提出的脊髓损伤多靶区神经环路磁刺激方案,已在临床上获得积极的下肢运动康复效果,但是其作用机制尚不明确。实验对T10压迫脊髓不完全损伤大鼠模型进行连续3周的左侧运动皮质及双侧L5神经根的神经环路磁刺激,发现损伤脊髓中星形胶质细胞标志物胶质纤维酸性蛋白表达下降,炎症因子白细胞介素1β、白细胞介素6和肿瘤坏死因子α表达减少,神经存活标志物微管相关蛋白2和突触可塑性标志物突触后致密蛋白95及突触素蛋白表达增加,皮质脊髓束的下行传导功能明显改善,运动功能显著恢复。表明双靶区神经环路磁刺激减弱星形胶质细胞活化改善了运动功能,为双靶区神经环路磁刺激应用于脊髓损伤临床治疗提供了理论依据。

https://orcid.org/0000-0002-3298-1072 (Qing Xie); https://orcid.org/0000-0002-8477-5377 (Dong-Sheng Xu)

关键词: 脊髓损伤, 神经环路磁刺激, 神经调节技术, 运动功能, 运动诱发电位, 星形胶质细胞, 炎症反应, 神经修复, 微管相关蛋白2, 突触可塑性

Abstract: Multi-target neural circuit-magnetic stimulation has been clinically shown to improve rehabilitation of lower limb motor function after spinal cord injury. However, the precise underlying mechanism remains unclear. In this study, we performed double-target neural circuit-magnetic stimulation on the left motor cortex and bilateral L5 nerve root for 3 successive weeks in a rat model of incomplete spinal cord injury caused by compression at T10. Results showed that in the injured spinal cord, the expression of the astrocyte marker glial fibrillary acidic protein and inflammatory factors interleukin 1β, interleukin-6, and tumor necrosis factor-α had decreased, whereas that of neuronal survival marker microtubule-associated protein 2 and synaptic plasticity markers postsynaptic densification protein 95 and synaptophysin protein had increased. Additionally, neural signaling of the descending corticospinal tract was markedly improved and rat locomotor function recovered significantly. These findings suggest that double-target neural circuit-magnetic stimulation improves rat motor function by attenuating astrocyte activation, thus providing a theoretical basis for application of double-target neural circuit-magnetic stimulation in the clinical treatment of spinal cord injury. 

Key words: astrocyte, inflammatory response, microtubule-associated protein 2, motor function, motor-evoked potentia, neural circuit-magnetic stimulation, neural repair, neuromodulation technique, spinal cord injury, synaptic plasticity