中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (15): 1453-1459.doi: 10.4103/1673-5374.139462

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

丹红注射液预处理脑缺血再灌注损伤的效应

  

  • 收稿日期:2014-06-06 出版日期:2014-08-12 发布日期:2014-08-12
  • 基金资助:

    中国国家自然科学基金(81173592),中国国家科技重大专项课题(2011ZX09201-201, 2012ZX09101201-004, 2012zx09101202, NCET-13-0935, 2013ZX09201020),天津市应用基础与前沿技术研究计划(14JCYBJC28900),天津市大学创新团队培训计划(TD12-5035)

Pretreatment with Danhong injection protects the brain against ischemia-reperfusion injury

Shaoxia Wang 1, Hong Guo 2, Xumei Wang 1, Lijuan Chai 1, Limin Hu 1, Tao Zhao 3, Buchang Zhao 3, Xiaoxu Tan 2, Feifei Jia 2   

  1. 1 Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China
    2 Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin, China
    3 Buchang Pharmaceutical Co., Ltd., Xi’an, Shaanxi Province, China
  • Received:2014-06-06 Online:2014-08-12 Published:2014-08-12
  • Contact: Limin Hu, Ph.D., Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China, huliminth@126.com.

摘要:

丹红注射液是有中国传统药物丹参和红花中提取制成的中药制剂,在中国被广泛用于治疗急性缺血性卒中,实验拟探讨其作用机制。以腹腔注射0.9,1.8mL/kg丹红注射液预处理脑缺血再灌注损伤模型大鼠,结果显示大鼠脑梗死面积明显减少,神经功能缺损评分明显下降,损伤血脑屏障通透性降低,紧密连接蛋白occludin表达增加,嗜中性粒细胞浸润和基质金属蛋白酶9表达减少,而基质金属蛋白酶2表达没有变化。且在损伤后3h腹腔注射1.8mL/kg丹红注射液治疗,也能降低神经功能缺损评分,减少脑梗死体积。提示丹红注射液对脑缺血再灌注损伤的神经保护效果是通过保护血脑屏障及逆转中性粒细胞浸润而实现的。

关键词: 神经再生, 丹红注射液, 丹参, 红花, 脑缺血再灌注, 中性粒细胞浸润, 基质金属蛋白酶, 血脑屏障

Abstract:

Danhong injection (DHI), a Chinese Materia Medica standardized product extracted from Radix Salviae miltiorrhizae and Flos Carthami tinctorii, is widely used in China for treating acute ischemic stroke. In the present study, we explored the neuroprotective efficacy of DHI in a rat model of temporary middle cerebral artery occlusion, and evaluated the potential mechanisms underlying its effects. Pretreatment with DHI (0.9 and 1.8 mL/kg) resulted in a significantly smaller infarct volume and better neurological scores than pretreatment with saline. Furthermore, DHI significantly reduced the permeability of the blood-brain barrier, increased occludin protein expression and decreased neutrophil infiltration, as well as profoundly suppressing the upregulation of matrix metallopeptidase-9 expression seen in rats that had received vehicle. Matrix metallopeptidase-2 expression was not affected by ischemia or DHI. Moreover, DHI (1.8 mL/kg) administered 3 hours after the onset of ischemia also improved neurological scores and reduced infarct size. Our results indicate that the neuroprotective efficacy of DHI in a rat model of cerebral ischemia-reperfusion injury is mediated by a protective effect on the blood-brain barrier and the reversal of neutrophil infiltration.

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