中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (15): 1446-1452.doi: 10.4103/1673-5374.139461

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

灵芝预处理对脑缺血再灌注损伤海马神经元保护效应

  

  • 收稿日期:2014-05-05 出版日期:2014-08-12 发布日期:2014-08-12

Neuroprotective effect of pretreatment with ganoderma lucidum in cerebral ischemia/reperfusion injury in rat hippocampus

Wangxin Zhang 1, 2, Qiuling Zhang 2, Wen Deng 2, Yalu Li 2, Guoqing Xing 2, Xianjun Shi 2, Yifeng Du 1   

  1. 1 Department of Neurology, Shandong Provincial Hospital, Shandong University, Jinan, Shandong Province, China
    2 Department of Medical Psychology, Taishan Medical University, Taian, Shandong Province, China
  • Received:2014-05-05 Online:2014-08-12 Published:2014-08-12
  • Contact: Yifeng Du, M.D, Department of Neurology, Shandong Provincial Hospital, Shandong University, Jinan 250021, Shandong Province, China, duyifeng2013@163.com.
  • Supported by:

    This work was supported by the Natural Science Foundation of Taishan Medical University in China, No. 2007.ZR-087.

摘要:

药理研究发现,传统中药灵芝具有较强的抗氧化及抗炎作用,能显著降低脑梗死面积,并减少缺血皮质神经元的凋亡。为此,设计了预先以灵芝水溶液灌胃治疗大鼠脑缺血再灌注损伤的实验方案,旨在验证以灵芝预处理对缺血再灌注损伤脑神经元的保护效应及机制。结果发现灵芝预处理3d和7d后脑缺血再灌注损伤大鼠海马神经元缺失减少,海马和血清中丙二醛水平降低,海马组织中炎症相关因子肿瘤坏死因子α和白细胞介素8表达降低,海马和血清中超氧化物歧化酶水平提升。表明灵芝通过抗氧化和抗炎作用对脑缺血再灌注损伤大鼠海马神经元产生了保护效应。

关键词: 神经再生, 脑缺血再灌注, 灵芝, 抗氧化, 抗炎, 超氧化物歧化酶, 丙二醛, 白细胞介素8, 瘤坏死因子α, 细胞凋亡, 海马

Abstract:

Ganoderma lucidum is a traditional Chinese medicine, which has been shown to have both anti-oxidative and anti-inflammatory effects, and noticeably decreases both the infarct area and neuronal apoptosis of the ischemic cortex. This study aimed to investigate the protective effects and mechanisms of pretreatment with ganoderma lucidum (by intragastric administration) in cerebral ischemia/reperfusion injury in rats. Our results showed that pretreatment with ganoderma lucidum for 3 and 7 days reduced neuronal loss in the hippocampus, diminished the content of malondialdehyde in the hippocampus and serum, decreased the levels of tumor necrosis factor-α and interleukin-8 in the hippocampus, and increased the activity of superoxide dismutase in the hippocampus and serum. These results suggest that pretreatment with ganoderma lucidum was protective against cerebral ischemia/reperfusion injury through its anti-oxidative and anti-inflammatory actions.