中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (12): 1989-1996.doi: 10.4103/1673-5374.172317

• 观点:神经损伤修复保护与再生 • 上一篇    下一篇

可能成为红景天苷对局灶性脑缺血再灌注损伤发挥神经保护作用靶点的Nrf2通路

  

  • 收稿日期:2015-10-12 出版日期:2015-12-30 发布日期:2015-12-30
  • 基金资助:

    福建中医院独立研究项目（2012fjzyyk-4）、福建省自然科学基金（2014j01340），福建省卫生和计划生育委员会研究项目（2014-zqn-jc-32）

Neuroprotective effects of salidroside on focal cerebral ischemia/reperfusion injury involve the nuclear erythroid 2-related factor 2 pathway

Jing Han1, Qing Xiao1, Yan-hua Lin1, Zhen-zhu Zheng1, Zhao-dong He1, Juan Hu1, 2, *, Li-dian Chen2, *   

  1. 1 Institute of Materia Medica, Fujian Academy of Traditional Chinese Medicine, Fuzhou, Fujian Province, China
    2 Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian Province, China
  • Received:2015-10-12 Online:2015-12-30 Published:2015-12-30
  • Contact: Juan Hu, Ph.D. or Li-dian Chen, Ph.D., huj@fjtcm.edu.cn or lidianchen87@yahoo.com.
  • Supported by:

    This work was supported by the Independent Research Project of Fujian Academy of Traditional
    Chinese Medicine in China, No. 2012fjzyyk-4; the Natural Science Foundation of Fujian Province in China, No. 2014J01340; the Research Project of Fujian Provincial Health and Family Planning Commission, No. 2014-ZQN-JC-32; a grant from the Platform for Preclinical Studies of Traditional Chinese Medicine and Quality Control Engineering Technology Research Center of Fujian Province in China, No. 2009Y2003.

PDF

575

摘要：

中药红景天的主要有效成分红景天苷已被证明对于脑缺血损伤具有显著的神经保护作用，但其作用机制尚不明确。为此，实验在大鼠MCAO模型基础上观察了红景天苷对脑缺血所致氧化应激损伤作用过程中NF-E2相关因子(nuclear factor erythroid 2-related factor 2，Nrf2)通路的变化。结果表明，红景天苷(30 mg/kg)尾静脉注射可显著减少模型大鼠脑梗塞灶大小，改善其神经功能并减轻脑组织病理学损伤，上调脑组织超氧化物歧化酶和谷胱甘肽S-转移酶活性，降低丙二醛含量，并能红景天苷显著增加脑组织Nrf2及其下游HO-1的表达。结果证实，红景天苷通过激活Nrf2通路发挥抗氧化作用，说明以此对脑缺血再灌注损伤的发挥神经保护作用，Nrf2通路可能成为红景天苷治疗脑缺血的更有效的可作用靶点。

关键词: 神经再生, 中医药, 红景天苷, 红景天, 脑缺血再灌注损伤, 抗氧化, 核因子NF-E2相关因子2, 血红素加氧酶1, MCAO模型, 谷胱甘肽-S-转移酶, 超氧化物歧化酶, 丙二醛, 神经保护

Abstract:

Salidroside, the main active ingredient extracted from Rhodiola crenulata, has been shown to be neuroprotective in ischemic cerebral injury, but the underlying mechanism for this neuroprotection is poorly understood. In the current study, the neuroprotective effect of salidroside on cerebral ischemia-induced oxidative stress and the role of the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway was investigated in a rat model of middle cerebral artery occlusion. Salidroside (30 mg/kg) reduced infarct size, improved neurological function and histological changes, increased activity of superoxide dismutase and glutathione-S-transferase, and reduced malon-dialdehyde levels after cerebral ischemia and reperfusion. Furthermore, salidroside apparently increased Nrf2 and heme oxygenase-1 expression. These results suggest that salidroside exerts its neuroprotective effect against cerebral ischemia through anti-oxidant mechanisms and that activation of the Nrf2 pathway is involved. The Nrf2/antioxidant response element pathway may become a new therapeutic target for the treatment of ischemic stroke.

Key words: nerve regeneration, traditional Chinese medicine, salidroside, cerebral ischemia and reperfusion, nuclear factor erythroid 2-related factor 2, heme oxygenase-1, middle cerebral artery occlusion model, superoxide dismutase, neuroprotection, neural regeneration