中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (2): 260-265.doi: 4103/1673-5374.152380

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

人羊膜上皮细胞移植修复臂丛神经损伤：黏弹性评价

  

  • 收稿日期:2015-01-20 出版日期:2015-02-17 发布日期:2015-02-17
  • 基金资助:

    吉林省科技发展计划资助项目（20110492）

Human amniotic epithelial cell transplantation for the repair of injured brachial plexus nerve: evaluation of nerve viscoelastic properties

Hua Jin 1, Qi Yang 2, Feng Ji 3, Ya-jie Zhang 1, Yan Zhao 1, Min Luo 2   

  1. 1 Jinan Maternity and Child Care Hospital, Jinan, Shandong Province, China
    2 China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China
    3 Shandong Corps Hospital of Chinese People’s Armed Police Forces, Jinan, Shandong Province, China
  • Received:2015-01-20 Online:2015-02-17 Published:2015-02-17
  • Contact: Feng Ji, 365883787@qq.com
  • Supported by:

     This study was financially supported by grants from the Science and Technology Development Plan Program of Jilin Province of China, No. 20110492.

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摘要：

 研究已证实胚胎干细胞移植能有效改善动物损伤周围神经的蠕变性能，由于羊膜上皮细胞也具有类似胚胎干细胞的生物学特性，作者推测羊膜上皮细胞移植修复损伤周围神经，能够在一定程度上恢复臂丛神经的蠕变性能。实验以牵拉神经根的方法制备C6臂丛神经损伤家兔模型后即刻，将人羊膜上皮细胞悬液注入C6臂丛神经损伤区头尾侧4.0 mm处(1×106/mL，3 μL/点)，26周后发现模型组家兔C6臂丛神经的7200s应力下降量、7200s应变上升量增大，臂丛神经黏弹性应力松弛、蠕变特性明显恢复，且前爪功能显著改善。提示人羊膜上皮细胞移植修复家兔损伤臂丛神经效果明显，以黏弹性指标判定动物损伤臂丛神经修复效果是评价的重要指标之一。

关键词: 神经再生, 臂丛神经损伤, 人羊膜上皮细胞, 前爪功能, 应力松弛, 蠕变, 黏弹性

Abstract:

The transplantation of embryonic stem cells can effectively improve the creeping strength of nerves near an injury site in animals. Amniotic epithelial cells have similar biological properties as embryonic stem cells; therefore, we hypothesized that transplantation of amniotic epithelial cells can repair peripheral nerve injury and recover the creeping strength of the brachial plexus nerve. In the present study, a brachial plexus injury model was established in rabbits using the C6 root avulsion method. A suspension of human amniotic epithelial cells was repeatedly injected over an area 4.0 mm lateral to the cephal and caudal ends of the C6 brachial plexus injury site (1 × 106 cells/mL, 3 μL/injection, 25 injections) immediately after the injury. The results showed that the decrease in stress and increase in strain at 7,200 seconds in the injured rabbit C6 brachial plexus nerve were mitigated by the cell transplantation, restoring the viscoelastic stress relaxation and creep properties of the brachial plexus nerve. The forepaw functions were also significantly improved at 26 weeks after injury. These data indicate that transplantation of human amniotic epithelial cells can effectively restore the mechanical properties of the brachial plexus nerve after injury in rabbits and that viscoelasticity may be an important index for the evaluation of brachial plexus injury in animals.

Key words: nerve regeneration, brachial plexus injury, human amniotic epithelial cells, forepaw function, stress relaxation, creep, viscoelasticity, neural regeneration