中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (7): 1101-1106.doi: 10.4103/1673-5374.160103

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

大鼠大脑硬脑膜中的新型年龄相关网络结构

  

  • 收稿日期:2015-06-10 出版日期:2015-07-24 发布日期:2015-07-24
  • 基金资助:

    韩国教育部国家研究基金国际博士奖学金计划(2014H1A2A1020588)，韩国原子能研究所基金，韩国药物针刺研究所基金(KPI-2014-010)，韩国KIOM基金K13290，韩国科学、ICT和未来计划部国家研究基金基础科学研究项目(2013R1A1A2021577)

Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain

Ho-Sung Lee 1, 2, Dai-In Kang 3, Seung Zhoo Yoon 4, Yeon Hee Ryu 5, Inhyung Lee 6, Hoon-Gi Kim 7, Byung-Cheon Lee 1, Ki Bog Lee 8   

  1. 1 Ki Primo Research Laboratory, Division of Electrical Engineering, KAIST Institute for Information Technology Convergence, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea
    2 Nadi Primo Research Institute, Graduate School of Integrative Medicine, Sun Moon University, Asan-si, Korea
    3 Pharmacopuncture Medical Research Center, Korean Pharmacopuncture Institute, Seoul, Korea
    4 Department of Anesthesiology and Pain Medicine, College of Medicine, Korea University, Seoul, Korea
    5 Acupuncture, Moxibustion & Meridian Research Group, Medical Research Division, Korea Institute of Oriental Medicine, Seoul, Korea
    6 Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    7 Faculty of Liberal Education, Seoul National University, Seoul, Korea
    8 Korea Atomic Energy Research Institute, Daejeon, Korea
  • Received:2015-06-10 Online:2015-07-24 Published:2015-07-24
  • Contact: Ki Bog Lee, Ph.D. or Byung-Cheon Lee, Ph.D., kblee@kaeri.re.kr or donboscolee@gmail.com.
  • Supported by:

    This research was supported by Global Ph.D. Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2014H1A2A1020588), by Korea Atomic Energy Research Institute, by a Korean Pharmacopuncture Foundation Grant funded by the Korean Pharmacopuncture Institute (KPI-2014-010), by the grant K13290 of KIOM and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2013R1A1A2021577).

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摘要：

应用铬-苏木精染色，我们在大鼠大脑硬脑膜中找到了新型年龄相关的网络结构存在的证据。在立体显微镜下，我们注意到铬-苏木精染色的线样结构，其存在于一定年龄段的大鼠特定脑区中，如4周龄大鼠的大脑侧叶和小脑中，而在1周龄的大脑脑中则几乎未见。在7周龄的大鼠脑中，这些结构变得更大、网络化更为明显。在相差显微镜下，我们发现1周龄大鼠大脑特定脑区中铬-苏木精染色颗粒散在分布，在4和7周龄大鼠的这些特定脑区中这些颗粒进一步形成网络结构。应用光镜和透射电镜观察到：随着年龄的增长，散布的颗粒融合成网络；年龄相关网络结构的切片呈重染嗜碱亚结构；透射电镜显示该嗜碱亚结构呈现出由纳米颗粒组成的高电子密度集簇。这些证据证实了硬脑膜作为普遍意义上的支持结构外，存在年龄相关的网络结构。

关键词: 硬脑膜, 铬-苏木精染色, 筋膜, 第一血管系统, 大脑, 激素

Abstract:

With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-week-old rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix. 

Key words: nerve regeneration, dura mater, chromium-hematoxylin staining, fascia, primo vascular system, brain, hormone, neural regeneration