中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (3): 512-518.doi: 10.4103/1673-5374.179078

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

一种新方法提高去细胞神经修复周围神经损伤的效果

  

  • 收稿日期:2015-12-22 出版日期:2016-03-15 发布日期:2016-03-15

Cartilage oligomeric matrix protein enhances the vascularization of acellular nerves

Wei-ling Cui 1, Long-hai Qiu 2, Jia-yan Lian 1, Jia-chun Li 2, Jun Hu , Xiao-lin Liu 2   

  1. 1 Department of Endocrinology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China
    2 Department of Orthopaedics and Microsurgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China
  • Received:2015-12-22 Online:2016-03-15 Published:2016-03-15
  • Contact: Jun Hu, Ph.D.,hjhjun@163.com.
  • Supported by:

    This study was supported by the Specialized Research Fund for Science and Technology Plan of Guangdong Province in China, No. 201313060300007; the National High-Technology Research and Development Program of China (863 Program), No. 2012AA020507; the National Basic Research Program of China (973 Program), No. 2014CB542201; the Doctoral Program of Higher Education of China, No. 20120171120075; and Doctoral Start-up Project of the Natural Science Foundation of Guangdong Province in China, No. S201204006336 and 1045100890100590.

摘要:

有研究表明,去细胞神经的血管化可提高桥接修复损伤神经的能力,作为去细胞基质材料的寡居硫酸软骨素可促进血管生成,提高修复损伤去细胞神经的血管化能力?为此,我们构建大鼠坐骨神经10 mm缺损模型,随机分为2组,对照组采用单纯去细胞神经修复法干预,实验组采用去细胞神经修复+腹腔注射寡居硫酸软骨素-促血管生成素1联合干预。二维放射显影法检测,发现术后7 d两组大鼠血管从吻合口两端长入,术后21 d移植体完全覆盖血管。与对照组相比,实验组大鼠血管密度和面积和血管化速度均增加。结果验证,寡居硫酸软骨素具有提高修复去细胞神经的血管化能力。

关键词: 神经再生, 周围神经损伤, 去细胞神经, 放射显影法, 神经修复, 神经组织工程, 二维评价, 血管化模型, 血管再生, 血管化

Abstract:

Vascularization of acellular nerves has been shown to contribute to nerve bridging. In this study, we used a 10-mm sciatic nerve defect model in rats to determine whether cartilage oligomeric matrix protein enhances the vascularization of injured acellular nerves. The rat nerve defects were treated with acellular nerve grafting (control group) alone or acellular nerve grafting combined with intraperitoneal injection of cartilage oligomeric matrix protein (experimental group). As shown through two-dimensional imaging, the vessels began to invade into the acellular nerve graft from both anastomotic ends at day 7 post-operation, and gradually covered the entire graft at day 21. The vascular density, vascular area, and the velocity of revascularization in the experimental group were all higher than those in the control group. These results indicate that cartilage oligomeric matrix protein enhances the vascularization of acellular nerves.

Key words: nerve regeneration, peripheral nerve injuries, acellular nerves, radiography, nerve repair, nerve tissue engineering, two-dimensional evaluation, vascularized models, angiogenesis, neural regeneration