中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (2): 219-224.doi: 10.4103/1673-5374.152374

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

脊髓损伤大鼠早期椎板减压后神经功能恢复:抑制炎症因子可达到的效果?

  

  • 收稿日期:2014-11-13 出版日期:2015-02-17 发布日期:2015-02-17
  • 基金资助:

    2009年安徽省卫生厅医学科研课题(09C33);2014年度皖南医学院重点科研项目培育基金(WK2014ZF14)

Inhibition of inflammatory cytokines after early decompression may mediate recovery of neurological function in rats with spinal cord injury

Jia-bing Xie, Xin Zhang, Quan-hui Li, Zhu-jun Xu   

  1. Department of Trauma Orthopedics, Yijishan Hospital Affiliated to Wannan Medical College, Wuhu, Anhui Province, China
  • Received:2014-11-13 Online:2015-02-17 Published:2015-02-17
  • Contact: Zhu-jun Xu, wh4305@vip.sina.com
  • Supported by:

    This study was supported by a grant from the Anhui Provincial Health Department- Funded Medical Research Project in 2009 in China, No. 09C33; a grant from the Key Scientific Research Project of Cultivating Fund of Wannan Medical College in China, No. WK2014ZF14.

摘要:

多种炎症因子参与脊髓损伤,影响脊髓神经功能恢复。实验在环扎法建立脊髓静态压迫模型后8或72h进行椎板减压,观察减压后脊髓组织中炎症因子肿瘤坏死因子α的表达及大鼠神经功能的改善。结果发现进行减压的大鼠脊髓组织病理形态和行为能力明显改善,脊髓组织中炎症因子肿瘤坏死因子α表达和凋亡细胞数量下降,且8h减压的效果优于72h。结果提示早期椎板减压可产生抑制肿瘤坏死因子α表达的效果,或可成为改善脊髓损伤后神经功能的干预新靶点。
 

关键词: 神经再生, 脊髓损伤, 手术减压, 肿瘤坏死因子α, 细胞凋亡, 神经功能

Abstract:

A variety of inflammatory cytokines are involved in spinal cord injury and influence the recovery of neuronal function. In the present study, we established a rat model of acute spinal cord injury by cerclage. The cerclage suture was released 8 or 72 hours later, to simulate decompression surgery. Neurological function was evaluated behaviorally for 3 weeks after surgery, and tumor necrosis factor α immunoreactivity and apoptosis were quantified in the region of injury. Rats that underwent decompression surgery had significantly weaker immunoreactivity of tumor necrosis factor α and significantly fewer apoptotic cells, and showed faster improvement of locomotor function than animals in which decompression surgery was not performed. Decompression at 8 hours resulted in significantly faster recovery than that at 72 hours. These data indicate that early decompression may improve neurological function after spinal cord injury by inhibiting the expression of tumor necrosis factor α.

Key words: nerve regeneration, spinal cord injury, surgical decompression, tumor necrosis factor α, cell apoptosis, neurological function, neural regeneration