中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (2): 308-313.doi: 4103/1673-5374.152387

• 原著:退行性病与再生 • 上一篇    下一篇

螺旋藻多糖保护帕金森病模型小鼠的途径:抑制黑质多巴胺能神经元凋亡

  

  • 收稿日期:2015-01-19 出版日期:2015-02-17 发布日期:2015-02-17
  • 基金资助:

    山东省自然科学基金(ZR2011HQ008, ZR2011HM044)

Protective effects of a polysaccharide from Spirulina platensis on dopaminergic neurons in an MPTP-induced Parkinson’s disease model in C57BL/6J mice

Fang Zhang 1, Jian Lu 2, Ji-guo Zhang 3, Jun-xia Xie 4   

  1. 1 Department of Pharmacology, Medical College of Qingdao University, Qingdao, Shandong Province, China
    2 Department of Pharmacy, Xianyang Central Hospital, Xianyang, Shaanxi Province, China
    3 Department of Pharmacology, Taishan Medical College, Taian, Shandong Province, China
    4 Department of Physiology, Medical College of Qingdao University, Qingdao, Shandong Province, China
  • Received:2015-01-19 Online:2015-02-17 Published:2015-02-17
  • Contact: Fang Zhang, Ph.D. or Jun-xia Xie, Ph.D.,qdzhangfang@126.com or jxiaxie@public.qd.sd.cn.
  • Supported by:

     This study was financially supported by grants from the Natural Science Foundation of Shandong Province of China, No. ZR2011HQ008, ZR2011HM044.

摘要:

为验证螺旋藻多糖可能对多巴胺能神经元具有保护作用。我们采用MPTP腹腔注射诱导建立帕金森病小鼠模型,在建模前连续10d给予螺旋藻多糖灌胃预处理。结果发现经螺旋藻多糖干预的帕金森病小鼠黑质中酶酪氨酸羟化酶和多巴胺转运蛋白免疫反应和mRNA表达增加,血清和中脑组织中超氧化物歧化酶和谷胱甘肽过氧化物酶活性增加,但血清和中脑组织中单胺氧化酶B活性不变。表明螺旋藻多糖抑制MPTP损伤小鼠多巴胺能神经元凋亡的保护作用与其抗氧化特性有关。

关键词: 神经再生, 螺旋藻多糖, 帕金森病, 多巴胺能神经元, MPTP, 抗氧化

Abstract:

The present study aimed to determine whether a polysaccharide obtained from Spirulina platensis shows protective effects on dopaminergic neurons. A Parkinson’s disease model was established through the intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in C57BL/6J mice. Prior to the MPTP injection, some mice were pretreated with intraperitoneal injections of a polysaccharide derived from Spirulina platensis once daily for 10 days. The results showed that the immunoreactive staining and mRNA expression of the dopamine transporter and tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis, in the substantia nigra, were significantly increased in mice pretreated with 800 mg/kg of the polysaccharide compared with those in MPTP-treated mice. The activities of superoxide dismutase and glutathione peroxidase in the serum and midbrain were also increased significantly in mice injected with MPTP after pretreatment with the polysaccharide from Spirulina platensis. By contrast, the activity of monoamine oxidase B in serum and midbrain maintained unchanged. These experimental findings indicate that the polysaccharide obtained from Spirulina platensis plays a protective role against the MPTP-induced loss of dopaminergic neurons in C57BL/6J mice, and that the antioxidative properties of this polysaccharide likely underlie its neuroprotective effect.

Key words: nerve regeneration, polysaccharide from Spirulina platensis, Parkinson’s disease, MPTP, dopaminergic neurons, antioxidation, neural regeneration