中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (7): 1134-1138.doi: 10.4103/1673-5374.160110

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

人脐血干细胞和脑源性神经营养因子修复损伤视神经:生物力学评价

  

  • 收稿日期:2015-06-17 出版日期:2015-07-24 发布日期:2015-07-24
  • 基金资助:

    吉林省科技发展计划资助项目(20110492)

Human umbilical cord blood stem cells and brain-derived neurotrophic factor for optic nerve injury: a biomechanical evaluation

Zhong-jun Zhang 1, Ya-jun Li 2, Xiao-guang Liu 1, Feng-xiao Huang 1, Tie-jun Liu 1, Dong-mei Jiang 1, Xue-man Lv 3, Min Luo 4   

  1. 1 Department of Mechanics, PLA Air Force Aviation University, Changchun, Jilin Province, China
    2 Mathematics School, Jilin University, Changchun, Jilin Province, China
    3 Department of Ophthalmology, China-Japan Friendship Hospital, Jilin University, Changchun, Jilin Province, China
    4 Department of Pain, China-Japan Friendship Hospital, Jilin University, Changchun, Jilin Province, China
  • Received:2015-06-17 Online:2015-07-24 Published:2015-07-24
  • Contact: Zhong-jun Zhang, 393000596@qq.com.
  • Supported by:

    This study was supported by a grant from Science and Technology Development Program of Jilin Province of China, No. 20110492.

摘要:

人脐血干细胞与脑源性神经营养因子移植修复损伤视神经的研究己取得一定进展,但生物力学指标分析尚属少见。实验建立视神经钳夹伤家兔模型,在损伤7d时,向玻璃体内一次性注射50μg脑源性神经营养因子或106人脐血干细胞?损伤30d时发现,以脑源性神经营养因子或人脐血干细胞治疗的视神经损伤家兔视神经拉伸最大载荷、最大应力、最大应变、弹性限度载荷、弹性限度应力、弹性限度应变等力学性能明显改善,且视神经超微结构损伤也明显减轻。说明人脐血干细胞和脑源性神经营养因子能有效修复损伤视神经,改善其生物力学性能,促进损伤后的恢复。

关键词: 神经再生, 视神经损伤, 人脐血干细胞, 脑源性神经营养因子, 力学特性

Abstract:

Treatment for optic nerve injury by brain-derived neurotrophic factor or the transplantation of human umbilical cord blood stem cells has gained progress, but analysis by biomechanical indicators is rare. Rabbit models of optic nerve injury were established by a clamp. At 7 days after injury, the vitreous body received a one-time injection of 50 μg brain-derived neurotrophic factor or 1 × 106 human umbilical cord blood stem cells. After 30 days, the maximum load, maximum stress, maximum strain, elastic limit load, elastic limit stress, and elastic limit strain had clearly improved in rabbit models of optical nerve injury after treatment with brain-derived neurotrophic factor or human umbilical cord blood stem cells. The damage to the ultrastructure of the optic nerve had also been reduced. These findings suggest that human umbilical cord blood stem cells and brain-derived neurotrophic factor effectively repair the injured optical nerve, improve biomechanical properties, and contribute to the recovery after injury.

Key words: nerve regeneration, optic nerve injury, human umbilical cord blood stem cells, brain-derived neurotrophic factor, biomechanical properties, neural regeneration