中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (8): 1292-1297.doi: 10.4103/1673-5374.162762

• 原著:退行性病与再生 • 上一篇    下一篇

中药石菖蒲单体对Aβ42诱导PC12细胞的保护

  

  • 收稿日期:2015-06-10 出版日期:2015-08-24 发布日期:2015-08-24
  • 基金资助:

    广东省科技计划项目(2010B060900085)

Protective effects of components of the Chinese herb grassleaf sweetflag rhizome on PC12 cells incubated with amyloid-beta42

Zi-hao Liang1, Xiao-hui Cheng1, Zhi-gang Ruan1, Han Wang1, Shan-shan Li1, Jing Liu1, Guo-ying Li1, Su-min Tian2   

  1. 1 Department of Anatomy and Histology, Basic Medical College, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China
    2 Department of Physiology, Basic Medical College, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China
  • Received:2015-06-10 Online:2015-08-24 Published:2015-08-24
  • Contact: Su-min Tian, guangyaosumin@sina.com.
  • Supported by:
    This study is financially supported by a grant from Guangdong Provincial Science and Technology Plan Program of China, No. 2010B060900085.
     

摘要:

中药石菖蒲主要成分为β-细辛脑和丁香酚,且两者均可穿越血脑屏障,发挥保护大脑神经元的作用。为进一步了解其神经保护机制,实验首先以不同浓度的1×10-10-1×10-5 mol/Lβ-细辛脑和1×10-10-1×10-5 mol/L丁香酚干预PC12细胞,均未发现细胞存活率受到影响。然后以1×10-6mol/Lβ-细辛脑和1×10-6mol/L丁香酚干预Aβ诱导的PC12细胞,发现细胞存活率明显增加,促凋亡基因Bax mRNA表达减少,而抑制凋亡基因Bcl-2 mRNA表达增加,且β-细辛脑和丁香酚组合物的效果更好。说明β-细辛脑和丁香酚可通过抑制凋亡保护PC12细胞,且混合物效果优于单独作用效果。

关键词: 神经再生, 中草药, 阿尔茨海默病, PC12细胞, Aβ, 石菖蒲, β-细辛脑, 丁香酚, 细胞凋亡

Abstract:

The major ingredients of grassleaf sweetflag rhizome are β-asarone and eugenol, which can cross the blood-brain barrier and protect neurons. This study aimed to observe the neuroprotective effects and mechanisms of β-asarone and eugenol, components of the Chinese herb grassleaf sweetflag rhizome, on PC12 cells. First, PC12 cells were cultured with different concentrations (between 1 × 10–10 M and 1 × 10–5 M) of β-asarone and eugenol. Survival rates of PC12 cells were not significantly affected. Second, PC12 cells incubated with amyloid-beta42, which reduced cell survival, were cultured under the same conditions (1 × 10–6 M β-asarone and eugenol). The survival rates of PC12 cells significantly increased, while expression levels of the mRNAs for the pro-apoptotic protein Bax decreased, and those for the anti-apoptotic protein Bcl mRNA increased. In addition, the combination of β-asarone with eugenol achieved better results than either component alone. Our experimental findings indicate that both β-asarone and eugenol protect PC12 cells through inhibiting apoptosis, and that the combination of the two is better than either alone.

Key words: