中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (9): 1421-1426.doi: 10.4103/1673-5374.163466

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

胶原-壳聚糖多孔支架与骨髓间充质干细胞移植保护缺血性脑卒中

  

  • 收稿日期:2015-07-30 出版日期:2015-09-28 发布日期:2015-09-28
  • 基金资助:

    陕西省科技发展项目(2012KCT-16)

Chitosan-collagen porous scaffold and bone marrow mesenchymal stem cell transplantation for ischemic stroke

Feng Yan1, 2, Wei Yue3, Yue-lin Zhang1, Guo-chao Mao1, Ke Gao4, Zhen-xing Zuo4, Ya-jing Zhang3, Hui Lu3   

  1. 1 Department of Neurosurgery, the Third Affiliated Hospital of Xi’an Jiaotong University; Shaanxi Provincial People’s Hospital, Xi’an, Shaanxi Province, China
    2 Department of Neurosurgery, the Fourth People’s Hospital of Shaanxi, Xi’an, Shaanxi Province, China
    3 Department of Neurology, Tianjin Huanhu Hospital, Tianjin, China
    4 Department of Neurosurgery, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province, China
  • Received:2015-07-30 Online:2015-09-28 Published:2015-09-28
  • Contact: Yue-lin Zhang, M.D., zhangyuelin68@163.com
  • Supported by:

    This study was funded by a grant from Shaanxi Provincial Support Project of Scientific Research Development Plan of China, No. 2012KCT-16

摘要:

摘要:实验设计了骨髓间充质干细胞与自制的胶原-壳聚糖支架材料复合物移植至缺血性脑卒中大鼠缺血坏死区,并与单纯骨髓间充质干细胞移植相比较其效果,移植14d时骨髓间充质干细胞与胶原-壳聚糖支架材料复合物移植治疗的大鼠神经功能明显恢复,且缺血组织及周围组织、海马齿状回和脑室管膜下区中出现血管内皮生长因子阳性表达以及nestin标记的神经前体细胞,同时BrdU标记的骨髓间充质干细胞中可见大量的神经胶质纤维酸性蛋白表达和少量的神经元特异烯醇化酶表达。表明骨髓间充质干细胞与生物支架载体复合物移植对缺血性脑卒中有神经保护作用,弥补了单纯骨髓间充质干细胞移植的不足。

关键词: 神经再生, 缺血性脑卒中, 胶原-壳聚糖支架, 骨髓间充质干细胞, 细胞移植, 细胞分化, 神经功能

Abstract:

In this study, we successfully constructed a composite of bone marrow mesenchymal stem cells and a chitosan-collagen scaffold in vitro, transplanted either the composite or bone marrow mesenchymal stem cells alone into the ischemic area in animal models, and compared their effects. At 14 days after co-transplantation of bone marrow mesenchymal stem cells and the hitosan-collagen scaffold, neurological function recovered noticeably. Vascular endothelial growth factor expression and nestin-labeled neural precursor cells were detected in the ischemic area, surrounding tissue, hippocampal dentate gyrus and subventricular zone. Simultaneously, a high level of expression of glial fibrillary acidic protein and a low level of expression of neuron-specific enolase were visible in BrdU-labeled bone marrow mesenchymal stem cells. These findings suggest that transplantation of a composite of bone marrow mesenchymal stem cells and a chitosan-collagen scaffold has a neuroprotective effect following ischemic stroke.

Key words: nerve regeneration, ischemic stroke, chitosan-collagen scaffold, bone marrow mesenchymal stem cells, cell transplantation, cell differentiation, neurological function, neural regeneration