中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (11): 1799-1808.doi: 10.4103/1673-5374.170302

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

白细胞介素10启动子基因多态性与中国人脑梗死发病的相关性

  

  • 收稿日期:2015-07-10 出版日期:2015-12-07 发布日期:2015-12-07

MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?

Xiao-li Min1, 2, Ting-yong Wang3, Yi Cao4, Jia Liu1, Jin-tao Li1, #, Ting-hua Wang1, * , #   

  1. 1 Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan Province, China
    2 Faculty of Clinical Medicine, Yunnan University of Traditional Chinese Medicine; the First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan Province, China
    3 School of Economics of Sichuan University, Chengdu, Sichuan Province, China
    4 Department of Neurosurgery, the Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China
  • Received:2015-07-10 Online:2015-12-07 Published:2015-12-07
  • Contact: Ting-hua Wang, M.D., Ph.D.,tinghua_neuron@263.net.
  • Supported by:

    This work was supported by grants from the National Natural Science Foundation of China, No. 81271358; Yunnan Science Foundation of China, No. 2013FZ199.

摘要:

为了揭示脑缺血再灌注损伤的分子机制,我们应用miRNA微阵列检测了局灶性脑缺血大鼠大脑皮质miRNA表达情况。应用生物信息学工具对基因本体功能分类和这些差异表达的miRNA基因靶向的信号通路进行系统分析。结果表明,局灶性脑缺血再灌注期miRNAs表达谱明显改变,共15个miRNAs上调,44个miRNAs下调。这些差异表达的miRNAs靶标基因主要参与代谢和细胞过程,其被称为miRNA-基因本体网络的hub节点。最相关的信号通路包括D-谷氨酰胺和D-谷氨酸代谢,肾素-血管紧张素系统,过氧物酶体和PPAR信号通路,膜泡运输SNARE相互作用,钙信号通路。实验结果提示,miRNAs在脑缺血再灌注损伤病理过程中有重要作用,理解miRNAs表达及功能可使我们对脑缺血再灌注损伤的分子机制有了进一步的阐述。

关键词: 神经再生, microRNA, 治疗靶点, 脑缺血再灌注, miRNAs表达谱, 生物信息学分析, 基因本体分析, KEGG通路

Abstract:

To determine the molecular mechanism of cerebral ischemia/reperfusion injury, we examined the microRNA (miRNA) expression profile in rat cortex after focal cerebral ischemia/reperfusion
injury using miRNA microarrays and bioinformatic tools to systematically analyze Gene Ontology (GO) function classifications, as well as the signaling pathways of genes targeted by these differentially expressed miRNAs. Our results show significantly changed miRNA expression profiles in the reperfusion period after focal cerebral ischemia, with a total of 15 miRNAs up-regulated
and 44 miRNAs down-regulated. Target genes of these differentially expressed miRNAs were mainly involved in metabolic and cellular processes, which were identified as hub nodes of a miRNA-GO-network. The most correlated pathways included D-glutamine and D-glutamate metabolism, the renin-angiotensin system, peroxisomes, the PPAR signaling pathway, SNARE interactions in vesicular transport, and the calcium signaling pathway. Our study suggests that miRNAs play an important role in the pathological process of cerebral ischemia/reperfusion injury. Understanding miRNA expression and function may shed light on the molecular mechanism
of cerebral ischemia/reperfusion injury.

Key words: nerve regeneration, microRNA, therapeutic target, cerebral ischemia/reperfusion injury, miRNA expression profiles, bioinformatics analysis, Gene Ontology analysis, molecular mechanism, KEGG pathway, neural regeneration