中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (11): 1809-1813.doi: 10.4103/1673-5374.170308

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

白细胞介素10启动子基因多态性与中国人脑梗死发病的相关性

  

  • 收稿日期:2015-08-11 出版日期:2015-12-07 发布日期:2015-12-07
  • 基金资助:

    国家自然科学基金（81171867）

Correlating interleukin-10 promoter gene polymorphisms with human cerebral infarction onset

Xin-hong Jiang1, Ke-xu Lin2, Yi-xian Zhang1, Rong-hua Chen3, Nan Liu1, 3, *   

  1. 1 Department of Rehabilitation, Union Hospital, Fujian Medical University, Fuzhou, Fujian Province, China
    2 Department of Emergency, Union Hospital, Fujian Medical University, Fuzhou, Fujian Province, China
    3 Department of Neurology, Union Hospital, Fujian Medical University, Fuzhou, Fujian Province, China
  • Received:2015-08-11 Online:2015-12-07 Published:2015-12-07
  • Contact: Nan Liu, M.D.,xieheliunan1984@sina.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81171867; a grant from the Key Research Program of Fujian Department of Science and Technology of China, No. 2011Y0027.

PDF

896

摘要：

中国作者有研究表明缺乏白细胞介素10会加剧炎症反应并严重影响脑缺血的预后，为验证此结果，我们假设白细胞介素10基因启动子区域最重要的3个位点-1082A/G，-819C/T和-592A/C位点单核苷酸基因多态性与中国人脑梗死的发病有关，采用扩增受阻突变系统－聚合酶链反应技术对181例脑梗死患者和115例健康对照者的白细胞介素10基因启动子区域-1082A/G，-819C/T和-592A/C位点核甘酸多态性进行分析，发现脑梗死组白细胞介素10-1082A/G基因型的分布频率，白细胞介素10-1082A等位基因频率与对照组比较均差异有显著性意义；脑梗死组白细胞介素10的A等位基因频率高于对照组，其中AA基因型发生脑梗塞的风险是AG基因型的2.031倍，与高血压病共同构成影响脑梗死发生率的独立危险因素；而两组白细胞介素10-819C/T和10-592C/A的基因型分布频率及等位基因频率比较差异无显著性意义。结果证实，白细胞介素10-1082A/G基因多态性与中国人脑梗死的发病存在一定关联，其中A等位基因频率增高与脑梗死发病有关。

关键词: 神经再生, 脑损伤, 白细胞介素10, 启动子, 基因多态性, 缺血性脑卒中, 遗传易感性, 免疫应答, 炎症, 缺血再灌注损伤, 国家自然科学基金

Abstract:

Evidence suggests that interleukin-10 (IL-10) deficiency exacerbates inflammation and worsens the outcome of brain ischemia. In view of the critical role of the single nucleotide polymorphic sites -1082 (A/G) and -819 (C/T) in the promoter region of the IL-10 gene, we hypothesized that they are associated with cerebral infarction morbidity in the Chinese Han population. We genotyped these allelic gene polymorphisms by amplification refractory mutation system-polymerase chain reaction methods in 181 patients with cerebral infarction (cerebral infarction group) and 115 healthy subjects (control group). We identified significant differences in genotype distribution and allele frequency of the IL-10-1082 A/G allele between cerebral infarction and control groups (χ2 = 6.643, P = 0.010). The IL-10-1082 A allele frequency was significantly higher in the cerebral infarction group (92.3%) than in the control group (86.1%) (P = 0.015). Moreover, cerebral infarction risk of the AA genotype was 2-fold higher than with the AG genotype (OR = 2.031, 95%CI: 1.134–3.637). In addition, AA genotype together with hypertension was the independent risk factor of cerebral infarction (OR = 2.073, 95%CI: 1.278–3.364). No statistical difference in genotype distribution or allele frequency of IL-10-819 C/T was found between cerebral infarction and control groups (P > 0.05). These findings suggest that the IL-10-1082 A/G gene polymorphism is involved in cerebral infarction, and increased A allele frequency is closely associated with occurrence of cerebral infarction.

Key words: neural regeneration, IL-10, promoter, gene polymorphisms, ischemic stroke, genetic susceptibility, inflammation, immune response, ischemia/ reperfusion injury, neural regeneration