中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (12): 2018-2024.doi: 10.4103/1673-5374.172321

• 原著:退行性病与再生 • 上一篇    下一篇

脐血与外周血G-CSF动员的单个核细胞移植有潜力治疗脑性瘫痪?

  

  • 收稿日期:2015-08-18 出版日期:2015-12-30 发布日期:2015-12-30
  • 基金资助:

    韩国Hanyang大学研究基金

Mononuclear cells from the cord blood and granulocyte- colony stimulating factor-mobilized peripheral blood: is there a potential for treatment of cerebral palsy?

Hani Koh1, Kyoujung Hwang2, Hae-Young Lim3, Yong-Joo Kim4, Young-Ho Lee4, 5, *   

  1. 1 Department of Translational Medicine, Graduate School of Biomedical Science & Engineering, Hanyang University, Seoul, Republic of Korea
    2 Greencrosscell Corp, Seoul, Republic of Korea
    3 Analytical Instrumentation Center Medical Branch, Hanyang University, Seoul, Republic of Korea
    4 Department of Pediatrics, Hanyang University College of Medicine, Seoul, Republic of Korea
    5 Cell Therapy Center, Hanyang University Medical Center, Seoul, Republic of Korea
  • Received:2015-08-18 Online:2015-12-30 Published:2015-12-30
  • Contact: Young-Ho Lee, M.D., Ph.D.,cord@hanyang.ac.kr.
  • Supported by:

    This work was supported by the Research Fund of Hanyang University (HY-2012).

摘要:

为探讨粒细胞集落刺激因子动员的外周血单个核细胞移植治疗脑性瘫痪的机制,我们对比分析了脑性瘫痪患儿与健康成人动员和未动员外周血单个核细胞及健康新生儿脐血单个核细胞炎性细胞因子及神经营养因子的表达。结果显示,动员和未动员外周血单个核细胞神经营养因子表达无明显差异,脑性瘫痪患儿经动员后外周血单个核细胞中促炎因子白细胞介素6表达较未动员单个核细胞明显升高,白细胞介素3表达明显降低,脑源性神经营养因子表达明显高于脐血或健康成人,外周血和脐血促炎因子白细胞介素1β, 白细胞介素3和白细胞介素6表达较健康成人明显降低,而抗炎因子白细胞介素8 和白细胞介素9表达则明显升高。说明脑性瘫痪患儿外周血和健康新生儿脐血动员后的单个核细胞有潜力成为细胞疗法治疗脑性瘫痪的种子细胞。

关键词: 神经再生, 神经营养因子, 炎症因子, 脐血, 粒细胞集落刺激因子, 外周血, 单个核细胞, 脑性瘫痪

Abstract:

To investigate a possible therapeutic mechanism of cell therapy in the field of cerebral palsy using granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cells (mPBMCs), we compared the expression of inflammatory cytokines and neurotrophic factors in PBMCs and mPBMCs from children with cerebral palsy to those from healthy adult donors and to cord blood mononuclear cells donated from healthy newborns. No significant differences in expression of neurotrophic factors were found between PBMCs and mPBMCs. However, in cerebral palsy children, the expression of interleukin-6 was significantly increased in mPBMCs as compared to PBMCs, and the expression of interleukin-3 was significantly decreased in mPBMCs as compared to PBMCs. In healthy adults, the expression levels of both interleukin-1β and interleukin-6 were significantly increased in mPBMCs as compared to PBMCs. The expression of brain-derived neurotrophic factors in mPBMC from cerebral palsy children was significantly higher than that in the cord blood or mPBMCs from healthy adults. The expression of G-CSF in mPBMCs from cerebral palsy children was comparable to that in the cord blood but significantly
higher than that in mPBMCs from healthy adults. Lower expression of pro-inflammatory cytokines (interleukin-1β, interleukin-3, and -6) and higher expression of anti-inflammatory cytokines (interleukin-8 and interleukin-9) were observed from the cord blood and mPBMCs from cerebral palsy children rather than from healthy adults. These findings indicate that mPBMCs from cerebral palsy and cord blood mononuclear cells from healthy newborns have the potential to become seed cells for treatment of cerebral palsy.

Key words: neurotrophic factors, inflammatory cytokines, cord blood, G-CSF mobilized peripheral blood, mononuclear cell, cerebral palsy, children, neural regeneration