中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (12): 2033-2039.doi: 10.4103/1673-5374.172323

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

电针如何促进脊髓损伤后前角运动神经元的功能恢复?

  

  • 收稿日期:2015-05-20 出版日期:2015-12-30 发布日期:2015-12-30
  • 基金资助:

    陕西省科技攻关项目(2003k09-03)

Electroacupuncture promotes the recovery of motor neuron function in the anterior horn of the injured spinal cord

Jian-hui Yang*, Jian-guo Lv, Hui Wang, Hui-yong Nie   

  1. Rehabilitation Center, First Affiliated Hospital of Health Science Center, Xi’an Jiaotong University, Xi’an, Shaanxi Province, China
  • Received:2015-05-20 Online:2015-12-30 Published:2015-12-30
  • Contact: Jian-hui Yang, hiuyang@sina.cn.
  • Supported by:

    This study was supported by a grant from the Shaanxi Province Scientific and Technological
    Project in China, No. 2014TM4193.

摘要:

针刺治疗急性脊髓损伤可以减轻损伤后炎症反应和脊髓的继发性损伤,但其作用机制尚不完全清楚。实验用改良Allen法压迫致伤T8, 9脊髓节段建立脊髓损伤大鼠模型,损伤后24 h分别电针刺激大鼠双下肢足三里(ST36)、悬种(GB39),伏兔(ST32)和三阴交(SP6)穴,并设单纯脊髓损伤大鼠(损伤)组作对照。RT-PCR,尼氏染色、酶组织化学染色、斜板试验和BBB评分结果显示,损伤后2,4,6周,与损伤组相比,电针组脊髓损伤区乙酰胆碱酯酶活性、脊髓前角大中型神经元的数目、胶质源性神经营养因子mRNA表达,BBB评分均有不同程度增加。结果证实电针可增加乙酰胆碱酯酶活性,并上调胶质源性神经营养因子表达,促进脊髓损伤后前角运动神经元功能的恢复。

关键词: 神经再生, 脊髓损伤, 中医药, 电针, 脊柱损伤, 脊髓继发性损伤, 穴位, 运动神经元, 乙酰胆碱酯酶, 胶质源性神经营养因子, 斜板试验, BBB评分法, 功能恢复

Abstract:

Acupuncture has been shown to lessen the inflammatory reaction after acute spinal cord injury and reduce secondary injury. However, the mechanism of action remains unclear. In this study, a rat model of spinal cord injury was established by compressing the T8–9 segments using a modified
Nystrom method. Twenty-four hours after injury, Zusanli (ST36), Xuanzhong (GB39), Futu (ST32) and Sanyinjiao (SP6) were stimulated with electroacupuncture. Rats with spinal cord injury alone were used as controls. At 2, 4 and 6 weeks after injury, acetylcholinesterase (AChE) activity at the site of injury, the number of medium and large neurons in the spinal cord anterior horn, glial cell line-derived neurotrophic factor (GDNF) mRNA expression, and Basso, Beattie and Bresnahan locomotor rating scale scores were greater in the electroacupuncture group compared with the control group. These results demonstrate that electroacupuncture increases AChE activity, up-regulates GDNF mRNA expression, and promotes the recovery of motor neuron function in the anterior horn after spinal cord injury.

Key words: nerve regeneration, spinal cord injury, electroacupuncture, spine injury, secondary injury, acupoint, motor neurons, acetylcholinesterase, glial cell line-derived neurotrophic factor, inclined board test, Basso, Beattie and Bresnahan locomotor rating scale, functional recovery, neural regeneration