中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (12): 2048-2053.doi: 10.4103/1673-5374.172325

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

聚氨酯聚乙烯醇水凝胶涂层可改良神经电极表面的细胞相容性

  

  • 收稿日期:2015-10-21 出版日期:2015-12-30 发布日期:2015-12-30
  • 基金资助:

    国家自然科学基金(81170768)

Polyurethane/poly(vinyl alcohol) hydrogel coating improves the cytocompatibility of neural electrodes

Mei Li1, Hai-han Zhou2, Tao Li3, Cheng-yan Li3, Zhong-yuan Xia1, *, Yanwen Y. Duan4, *   

  1. 1 Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China
    2 Institute of Molecular Science, Shanxi University, Taiyuan, Shanxi Province, China
    3 Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China
    4 College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei Province, China
  • Received:2015-10-21 Online:2015-12-30 Published:2015-12-30
  • Contact: Zhong-yuan Xia, M.D. or Yanwen Y. Duan, Ph.D., xiazhongyuan2005@aliyun.com or yduan@whu.edu.cn.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81170768; a grant from the Fundamental Research Project of Shanxi Province of China, No. 2015021079.

摘要:

作为神经假体核心部件神经电极多采用聚二甲基硅氧烷作为电极封装材料,而其植入后易引发组织炎性反应。鉴于聚氨酯、聚乙烯醇具有作为涂层材料的理化特性及良好生物相容性,我们合成了聚氨酯聚乙烯醇水凝胶涂层材料并以等离子预处理的方法将其涂覆于聚二甲基硅氧烷表面后与PC12细胞共培养,以提高其生物相容性。经蛋白吸附实验检测,涂覆后聚二甲基硅氧烷表面蛋白吸附量减少92%,且聚氨酯聚乙烯醇水凝胶涂覆的聚二甲基硅氧烷表面细胞密度,神经突触分化个数及突触长度均优于未涂覆的聚二甲基硅氧烷,显示了聚氨酯聚乙烯醇水凝胶具有较好的细胞相容性,有望作为神经电极的涂层材料改良其生物相容性。

关键词: 神经再生, 脑损伤, 神经电极, 水凝胶涂层, 聚二甲基硅氧烷, 聚乙烯醇, 聚氨酯, 细胞相容性, 神经生长因子, 蛋白吸附量, 大鼠肾上腺嗜铬细胞瘤细胞, 突触分化, 国家自然科学基金

Abstract:

Neural electrodes, the core component of neural prostheses, are usually encapsulated in polydimethylsiloxane (PDMS). However, PDMS can generate a tissue response after implantation. Based on the physicochemical properties and excellent biocompatibility of polyurethane (PU) and poly(vinyl alcohol) (PVA) when used as coating materials, we synthesized PU/PVA hydrogel coatings and coated the surface of PDMS using plasma treatment, and the cytocompatibility
to rat pheochromocytoma (PC12) cells was assessed. Protein adsorption tests indicated that the amount of protein adsorption onto the PDMS substrate was reduced by 92% after coating with the hydrogel. Moreover, the PC12 cells on the PU/PVA-coated PDMS showed higher cell density and longer and more numerous neurites than those on the uncoated PDMS. These results indicate that the PU/PVA hydrogel is cytocompatible and a promising coating material for neural electrodes to improve their biocompatibility.

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